摘要
目的:观察蛋白激酶C(prote in k inase C,PKC)在乙醇诱导人γδT细胞凋亡中的作用。方法:用结核杆菌多肽抗原(M tb-Ag)刺激正常人外周血单个核细胞,获得M tb-Ag激活的T细胞(γδT细胞为主);采用Annexin-V-FITC/PI染色流式细胞术观察不同浓度乙醇对人γδT细胞凋亡诱导情况,并观察Rottlerin(PKC抑制剂)预处理对乙醇诱导γδT细胞凋亡的影响。结果:4.0%的乙醇作用结核杆菌抗原活化的γδT细胞4 h、12 h、24 h均可显著诱导γδT细胞凋亡(P<0.01);5.0μmol/L和40.0μmol/L Rottlerin可显著抑制4.0%乙醇诱导γδT细胞凋亡(P<0.01),凋亡抑制率分别为75.17%和58.95%。结论:PKC途径参与乙醇诱导人γδT细胞凋亡信号传导。
Objective：To investigate the effect of protein kinase C （PKC） on the apoptosis of human γδT cells induced by ethanol. Methods; The peripheral blood mononuclear cells of healthy donors were stimulated with the peptide antigen derived from Mycobacterium tuberculosis antigen （Mtb-Ag）, to generate Mtb-Ag activated T cells （ γδT cells were predominant）. The apoptosis of human γδT cells induced by different concentrations of ethanol was measured by flowcytometry using Annexin-V-FITC/PI staining, and the effect of pretreatment with the PKC inhibitor Rottlerin on the apoptosis of human γδT cells induced by ethanol was also investigated. Results ：The apoptosis of activated human γδT cells stimulated with Mtb-Ag were significantly induced by treatment of γδT ceils with 4.0% ethanol for 4,12 and 24 hours（P 〈0.01）. The apoptosis of γδT cells induced by 4.0% ethanol were inhibited remarkably by 5.0 μmol/L and 40.0 μmol/L Rottlerin, the inhibitory rates of apoptosis were 75. 17% and 58.95% （ P 〈 0.01 ）, respectively. Concluslons：The results suggested the PKC signaling pathways participated in the apoptosis of γδT cells induced by ethanol.
出处
《蚌埠医学院学报》
CAS
2006年第3期230-232,共3页
Journal of Bengbu Medical College
基金
安徽省教育厅自然科学研究资助项目(2005KJ375ZC)
