摘要
本文报道了用表面增强傅里叶变换拉曼光谱法测定的脱氧核糖核酸（ＤＮＡ）水溶液及固态ＤＮＡ的拉曼散射结果。实验表明，由于银膜底物对水溶液中ＤＮＡ各基因的有效吸附，可在较低浓度下（１μｍ／ｍｌ）获得不受荧光干扰的拉曼光谱图，其频率和相对强度分布与所测固态ＤＮＡ拉曼光谱有明显的差异。其中，水溶液状态下吸附在银膜上的ＤＮＡ的核糖部分Ｃ＝Ｏ及碱基腺嘌呤基团有关振动谱带均得到显著增强。对水溶液ＤＮＡ的表面增强拉曼谱带的归属进行了讨论。
The Raman spectra of DNA obtained in aqueous solution and in solid state by surface enhanced Fourier transform Raman spectroscopy (SE--FT--RS) are presented. The experimental results show that Raman spectrum of DNA in aqueous solution without any fluorescence interference can be obtained at low concentration (1μg/ml) via adsorption groups of DNA on surface of Ag film. It is obvious that different patterns on shift and relative intensity distribution of DNA exist in aqueous solution and solid state. In aqueous solution f the Raman spectral bands of nucleic ribose C￣O groups and adenine groups are generally enhanced. The assignment of DNA bands in aqueous solution is discussed.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
1996年第3期60-65,共6页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金
关键词
表面增强
拉曼散射
银膜
SEFTRS
DNA
Deoxyribonucleic acid (DNA), Surface enhanced Raman scattering (SERS),Ag film
