摘要
用核磁共振(NMR)波谱、HPLC和化学定磷法研究了稀土元素对3＇-腺嘌呤核苷酸(3＇-AMP)及3＇-鸟嘌呤核苷酸(3＇-GMP)的水解断裂作用,结果表明:不同稀土元素对3＇位核苷酸断裂有较强的特异性,在37℃,pH9的条件下,使3＇-AMP断裂为腺嘌呤核苷(A)及无机磷,使3＇-GMP断裂为鸟嘌呤核苷(G)及无机磷,其断裂作用远大于5＇位核苷酸,并对其水解断裂进行了研究.
The hydrolytic cleavage of adenosine - 3' - monophosphate(3' - AMP) and guanosine -3' - monophosphate (3' - GMP) by lanthanides was investigated by NMR,HPLC and the method of measuring the liberated inorganic phosphate. The results show that lanthanides have specificity for hydrolyzing 3' - mononucleotides. 3' - AMP and 3' - GMP were converted to Adenosine(A),phosphate and Guanosine(G),phosphate respectively at pH 9,37℃ . The efficiency of cleavage was greater than that of 5'- mononucleotides. The mechanism of hydrolytic of cleavage was discussed in detail.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
1998年第1期47-51,共5页
Acta Chimica Sinica
基金
国家自然科学基金
中国科学院基础局资助项目(29631010)
关键词
稀土元素
3'-AMP
3’-GMP
水解断裂
断裂
lanthanide, adenosine - 3' - monophosphate, guanosine - 3' - monophosphate, hydrolytic cleavage
