摘要
目的:为快速、灵敏、简便的检测祛斑类化妆品中禁用物质氢醌、苯酚的含量,建立二极管阵列检测器高效液相色谱分析法。方法:用甲醇溶解,超声波提取样品后,在适当的色谱条件下,用紫外吸收谱图和保留时间定性测定。结果:该法线性良好,相关系数氢醌为r1=0.9997,苯酚r2=0.9999;氢醌回收率为94.5%-95.7%,苯酚回收率为98.8%-100%。结论:方法分离效果好,简便快捷,灵敏度高,重复性好,适用于各类化妆品中氢醌、苯酚测定,为首选方法。
Objective： To determine the hydroquinone and phenol in freckle removing cosmetics with high performance liquid chromatography （HPLC）, the method of diode array detector HPLC was established. Metheods： The sample was dissolved with methanol after ultrasonic extraction. In proper chromatographic conditions, the qualitative analysis was based on the retention time and UV graph.Results： The results showed that the method had great linearity.The respective corrdation of hydroquinone and phenol was 0.9997 and 0.9999 respectively.The recovery rate of hydroquinone was 94.5% - 95.7% and that of phenol was 98.8% - 100.0% .Conclusion： It mined out that the method had good separation, high sensitivity and good repetition. It could be the preferred method for determination of hydroquinone and phenol in freckle removing cosmetics.
出处
《现代预防医学》
CAS
北大核心
2006年第5期788-789,共2页
Modern Preventive Medicine
关键词
氢醌
苯酚
二极管阵列检测器
高效液相色谱法
化妆品
Hydroquinone, Phenol, Diode array detector, High performance liquid chromatograph
