摘要
为了研究超高压提取山楂叶中黄酮类化合物的最佳工艺,采用超高压技术常温提取,正交试验优化,分光光度法检测山楂叶中黄酮类化合物的含量。结果表明:超高压提取山楂叶中黄酮类化合物的最佳提取工艺参数为:提取溶剂为50%乙醇,提取压力为400 MPa,料液比为1∶45,提取温度为60℃,提取时间为3 m in。该超高压提取工艺具有提取效率高、时间短、杂质含量少等优点。
High hydrostatic pressure extraction （HHPE） technique was used to extract flavonoids from Hawthorn leaves at normal temperature. The content of flavonoids was determined by UV-spectrophotometry. Parameters that may influence the extraction process were investigated. Orthogonal test was applied to obtain the optimum extraction condition, which was as follows ： solid dissolved in 50% ethanol, ratio of raw Hawthorn leaves （g） to solvent （ml） of 1：45, temperature of 60℃, extraction pressure of 400 MPa and extraction time of 3 min. Results of this work show that HHPE possesses several advantages over other extraction methods, such as higher efficiency, shorter extraction time, and less impurity in the extracted flavonoids.
出处
《吉林大学学报（工学版）》
EI
CAS
CSCD
北大核心
2006年第3期438-442,共5页
Journal of Jilin University:Engineering and Technology Edition
基金
国家自然科学基金资助项目(30472135)
关键词
生物药物学
山楂叶
黄酮类化合物
超高压提取
正交实验
biological pharmacology
hawthorn leaves
flavonoids
high hydrostatic pressure extraction
orthogonal experimen
