摘要
目的探讨海马CA1区微血管LRP-1表达及微血管密度与认知功能改变的相关性。方法应用Morris水迷宫对不同月龄大鼠的认知功能进行测试;免疫组化技术测定大鼠海马CA1区神经细胞、微血管LRP-1表达,微血管Ⅷ因子相关抗原的表达,并应用MIAS图像分析系统进行平均光密度测定及微血管计数。结果①24月龄大鼠较3月龄和10月龄大鼠的游走距离显著延长。②3月龄和24月龄大鼠海马CA1区微血管LRP-1的表达显著高于神经细胞。随着月龄的增加,LRP-1在微血管的表达显著下降,组与组之间均有显著差异。③随着月龄的增加,微血管Ⅷ因子相关抗原的表达存在下降趋势,24月龄与3月龄大鼠比较差异具显著性,但24月龄与10月龄、10月龄与3月龄比较均无显著性差别;海马CA1区微血管计数各月龄组之间亦无显著差异。④随着月龄的增加,神经细胞LRP-1的表达亦存在下降趋势,24月龄与3月龄大鼠比较差异具显著性,但24月龄与10月龄、10月龄与3月龄比较均无显著性差异。⑤24月龄大鼠海马CA1区微血管LRP-1的表达与Marris水迷宫测试结果负相关,但海马CA1区神经细胞LRP-1的表达及Ⅷ因子相关抗原的表达与水迷宫测试结果不具明确的相关性。结论老龄大鼠认知功能下降与海马CA1区微血管LRP-1表达降低有显著相关性。
Objective To investigate the relationship between cognitive function with the LRP-1 or Ⅷ factor related antigen density in microvessels of hippceampal CA1 with aging. Methods Morris water maze was used to estimate the cognitive function of rats in various month-old. techniques were used to assess the expression of LRP-1 and Ⅷ factor related antigen of hippocampal CA1. Results The distance for 24-month-old rats to locate the hidden platform was significantly longer than 3-month-old and 10-month-old rats. Hippoeampal CA1 nerve cells and microvessels all had LRP-1 expression. With aging, the density of LRP-1 in microvessels was decreasing quickly, there was significant difference between each group. The density of LRP-1 in the nerve cells and Ⅷ factor related antigen was significantly decreased in 24-month-old rats, but there was no significant difference between the 3-month-old rats and the 10-month-old rats. The count of microvessels was not different between every two groups in hippocampal CA1. The decreased density of LRP-1 in the microvessels of the 24-month-old rats was correlated with the cognitive function impairment. Conclusion There is high correlation between the LRP-1 expression density in microvessels of hippocampal CA1 and the cognitive function impairment in aging rats.
出处
《中国微循环》
北大核心
2006年第2期95-98,i0001,共5页
Journal of Chinese Microcirculation
