摘要
目的建立高效液相色谱-荧光（HPLC-FL）法检测人血清中的双酚A。方法采用β-葡萄糖酸酐酶水解人血清中的结合型双酚A，乙醚-正己烷（1：1）混合溶液提取人血清中的双酚A，C18色谱柱分离，以己腈-四氢呋喃-水（40：3：60）的混合溶液为流动相，激发光波长227nm，发射光波长310nm检测人血清中的双酚A。结果双酚A在20min内完全洗脱出来，分离效果良好。在0～2500μg／L的浓度范围内，双酚A的质量浓度与峰面积成良好的线性关系，其在血清中的检测限为0．139μg／L。加标回收率大于83．5％，精密度为1．13％-5．26％。结论该法灵敏度高、重复性好，能够有效地分离和检测人血清中的双酚A，适用于人血清中双酚A的测定。
Objective To estabhsh a simple and highly sensitive HPLC method for determining bisphenol A in human serum. Method β-glucuronidase was used to hydrolyze bisphenol A in human serum samples, then the bisphenol A was extracted by a mixed solvent of n- hexane and diethyl ether （ 1 ： 1 ）, dried by nitrogen flow and dissolved with the mixture of acetonitrile and water （40： 60 ） ; the operating condition including C18 column, acetonitrile-tetrahydrofuran-water （40 ： 3 ： 60） mixture as mobile phase, a flow rate of 1 ml/min and fluorescence detector with 227 nm excitation wavelength and 310 nm emission wavelength. Result A complete elution of bisphenol A could be achieved in 20 minutes using the method mentioned above, and there was a good linear relationship between the concentrations of bisphenol A and their peak areas in the range of 0 - 2 500 μg/L. The detection limit in human serum was 0.139 μg/ L, the absolute recovery of blsphenol A in human serum was not less than 83.5%, and the inter-day precision was in the range of 1.13% to 5.26%. Conclusion The results showed that the method is pretty good both at sensitivity and repetition, which is reliable and suitable for the determination of bisphenol A in human serum.
出处
《中国工业医学杂志》
CAS
北大核心
2006年第2期70-73,共4页
Chinese Journal of Industrial Medicine
关键词
高效液相色谱法
双酚A
人血清
酶解
High-performance liquid chromatography
Bisphenol A
Human serum
Enzyme hydrolysis
