摘要
目的研究自体骨髓间充质干细胞(MSCs)移植对生长期山羊颅骨冠状缝牵张后组织改建的影响。方法选用处于发育期山羊10只,随机分为实验组与对照组,各5只。在颅骨冠状缝两侧安放自行研制的牵张器,以每天0．4mm的速率进行骨缝牵张,连续加力8 d。实验组动物骨缝牵张区给予注射自体骨髓间充质干细胞,对照组同期注射生理盐水。在牵张结束后第4周处死动物,取骨缝标本进行组织学和扫描电镜观察。结果所有实验动物的冠状缝均被成功牵开,骨缝边缘均可见新骨组织生成与改建。同对照组相比,实验组骨缝成骨与矿化较快,骨小梁分布密度及成熟程度也较高,骨缝形状已开始恢复正常。结论自体骨髓间充质干细胞移植对山羊颅骨缝牵张成骨可能有明显的促进作用。
Objective To observe the effects of mesenchymal stem cells （MSCs）on cranial suture under mechanical strain in growing goats. Methods 10 growing goats were used in this study. A customized distractor was used for distraction of the coronal suture at a rate of 0.4 mm/day for 8 days. The experimental group （5 goats） was injected with autologous MSCs into the distracted region, whereas the control group （5 goats） with injection of physiological saline. All animals were killed at 4 weeks after the end of distraction. Scanning electron microscopy and histological analysis were taken to observe the samples. Results 4 weeks after the end of distraction, the cranial sutures in all animals were separated successfully. The new bone formation at the edge of suture in the experimental group was superior to that in the control group. Conclusion Autologous MSCs transplantation may promote the cranial suture distraction osteogonesis in the srowing goats.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2006年第2期153-155,共3页
West China Journal of Stomatology
基金
国家自然科学基金资助项目(30271430)
四川省科技厅应用基础基金资助项目(03JY029-074-2)
关键词
张应力
骨缝牵张
新骨形成
mechanical strain
suture distraction osteogenesis
new bone formation
