摘要
目的:研究骨形成蛋白(bone morphogenetic protein,BMP)-2在犬面中份缝牵引成骨过程中的表达,探讨缝牵引成骨的机制。方法:18只12周龄杂种犬随机分为牵引成骨组12只和正常对照组6只,在牵引5、10、15d,固定10、20d,去固定1个月分别处死实验犬2只和对照犬1只,免疫组化法观察BMP-2在各骨缝的表达情况。结果:牵引期和固定期,BMP-2在实验犬各骨缝边缘大量增殖的未成熟成骨细胞、间充质细胞以及新生骨基质中呈阳性表达,对照犬也有少量表达。随着机械应力牵引,BMP-2在各骨缝表达量逐渐上升,在牵引15d阳性表达最强,以后逐渐下降,到去固定1个月时表达水平接近正常。结论:机械牵张力刺激可以导致内源性BMP-2产生,BMP-2可能在缝牵引新骨生成过程中扮演重要的角色。
Objective：To study the expression of bone morphogenetic protein （BMP）-2 during midfacial sutural distraction osteogenesis in dogs and to probe the mechanism of sutural distraction osteogenesis. Methods： Eighteen mongrel dogs （12-week old） were randomly divided into distraction osteogenesis group（n = 12） and the control group（ n = 6）. Rigid external distractor was used for sutural distraction of the midfacial skeleton in the experimental animals. Two experimental and one control dogs were sacrificed at defined time points （on the 5^th ,10^th and 15^th day of distraction, the 10^th and 20^th day of fixation, and 30^th day after completion of fixation）. The expression of BMP-2 was observed by means of immunohistochemistry. Results： During the period of distraction and fixation, positive staining of BMP-2 was found in the proliferated preosteoblasts, the mesenchymal cells and the newly formed bone matrix at the sutural margin of the experimental dogs; weak expression was also noticed in the control dogs. Compared with that in the control group, the expression of BMP-2 in the experimental group increased and reached the peak on the 15^th of distraction, then gradually decreased, the expression of BMP-2 nearly restored to the normal level 1 month after the removal of the distractor. Conclusion： The mechanical strain can lead to the generation of endogenous BMP-2, which may play an important role in the new bone formation
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2007年第12期1356-1360,共5页
Academic Journal of Second Military Medical University
基金
北京市自然科学基金(7072075)~~
关键词
骨形成蛋白2
面中份
缝牵引成骨术
免疫组织化学
bone morphogenetic protein-2
midface
suture distraction osteogenesis
immunohistochemistry
