摘要
利用半定量反转录聚合酶链反应(RT-PCR)克隆了黑龙江籽鹅IGF-Ⅰ基因,并检测了生长期间该基因mRNA在各组织中的表达量变化。结果表明,鹅IGF-Ⅰ基因与鸡、鸭IGF-Ⅰ基因的同源性分别为97.6%、99.3%;鹅肝组织中IGF-ⅠmRNA的表达水平在30日龄和60日龄较高,而30日龄的表达量极显著高于22、28日龄鹅胚和1日龄籽鹅,显著高于10日龄雏鹅;30日龄雏鹅IGF-ⅠmRNA在肝、卵巢、胸肌、肺、脾、腿肌、垂体、肌胃、肾、心等组织中都有表达,其中肝、卵巢、胸肌、肺的表达量较高。
An insulin-like growth factors-Ⅰ gene（IGF-Ⅰ ） from geese was cloned and its expression dynamics in tissues was examined by semi-quantitive RT-PCR using β-actin as an internal standard. The sequence similarities in IGF-Ⅰ gene between goose and chicken, and goose and duck were 97. 6% and 99.3%, respectively. The expression of IGF-Ⅰ in liver was more abundant on days 30 and 60 than on the other investigated days, and the expression of IGF I mRNA reached the highest on day 30. A positive correlation was established between expression of IGF-Ⅰ mRNA and weight gain. The IGF-Ⅰ mRNA was expressed in liver, ovary,breast,lung,spleen,leg muscle,pituitary,stomach,kidney and heart, and higher abundance of IGF-Ⅰ mRNA was found in liver,ovary,breast and lung.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2006年第3期216-219,共4页
Chinese Veterinary Science
