摘要
目的建立血浆血管性假血友病因子前肽(vWFpp)的测定方法,并检测152例健康体检者及36例冠心病患者和38例同龄正常对照组的血浆vWFpp水平。方法采用酶联免疫吸附分析(ELISA)双抗体夹心法对152例体检者和36例冠心病患者血浆vWFpp进行测定。结果152例体检者vWFpp水平为496.44±167.24μg/L。50～60岁年龄组vWFpp显著高于40岁以下年龄组(P<0.01)。男女间vWFpp水平差异无统计学意义(P>0.05)。男51～60岁年龄组与16～20,21～30,31～40岁的3个年龄组vWFpp水平差异都具有显著的统计学意义(P<0.05);152份体检者血浆样品vWFpp水平与年龄的相关性分析显示,vWFpp水平与年龄呈显著的正相关关系(r=0.444,P=0.000)。冠心病组vWFpp水平为1051.6±331.9μg/L,与同年龄段对照组vWFpp水平485.2±119.4μg/L比较具有显著性差异(P<0.001)。结论该法测定血浆vWFpp具有特异、快速、灵敏的优点,具有良好的临床应用前景。
Objective To establish a sandwich enzyme linked immunosorbent assay （ELISA） method for determination of von Willebrand factor propeptide （vWF-pp） in plasma and to determine thelevels of plasma vWF-pp in 152 healthy volunteers, 36 patients with coronary heart disease and 38 cases ofthe normal control group with the same ages. Methods The levels of vWF-pp in 152 healthy volunteers withdifferent ages and different genders and in 36 patients with coronary heart disease were determined with thesandwich enzyme linked immuno-sorbent assay （ELISA）. Results The average level of vWF-pp in 152healthy volunteers was 496.4±167. 2μg/L. The level of vWF-pp in the 50- 60 years old group wassignificantly higher than that in the less than 40 years old group （ P 〈 0. 05 ）. There was no significantdifferece between male and female （P 〉 0. 05 ）. There was significant difference between the 51-60 years old male group and the other three male groups respectively, the 16 ~20 years old group, the 21 -30 yearsold group and the 31 ~ 40 years old group （P 〈 0. 05 ） . There was the significant positive correlation betweenthe level of vWF-pp in 152 healthy volunteers and the age with r = 0. 444 and P = 0. 000. There wassignificant difference in the level of vWF-pp between the coronary heart disease groups （ 1051.6±331.9 ）μg/L） and the health groups （485.15±119.4）μg/L with the same ages. Conchmion The sandwichenzyme linked immunosorbent assay （ELISA） method is specific, rapid and sensitive in the determination ofthe plasma vWF-pp with a good perspective of the clinical application.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2006年第3期226-228,共3页
Chinese Journal of Laboratory Medicine
