摘要
目的检测药用胶囊中合成着色剂含量。方法高效液相色谱梯度洗脱法，流动相A为乙腈，B为0．02mol·L^-1乙酸铵，洗脱程序：0-5min，A相浓度8％-18％，B相浓度92％～82％；5～12min，A相浓度18％-74％，B相浓度82％～26％；12～15min，A相浓度74％-74％，B相浓度26％-26％。流动相流速为1．0mL·min^-1，紫外检测波长254nm，柱温25℃，进样量：10μL。结果药用胶囊中的合成着色剂回收率迭93．2％～98．5％，精密度RSD在0.8％-3．0％，浓度范围在5～60mg·L^-1时有良好的线性关系。结论应用此方法可使合成着色剂得到很好分离，测定结果满意。
OBJECTIVE To develop a high performance liquid chromatography （HPLC） for isolating anti measuring the content of synthetic colorants in officinal capsule. METHODS HPLC with gradient elution was used in the contents measurement of 6 different colors capsules. Mobile phase A was acetonitrile, B was 0.02 mol·L^-1 ammonium acetate. Elution program：0 - 5 min,8% - 18% of phase A and 92% - 82% of phase B; 5 - 12 min, 18% - 74% of phase A and 82% - 26% of phase B; 12 - 15 min, 74% - 74% of phase A and 26% - 26% of phase B. The elution speed of the mobile phase was 1.0mL·min^-1. The wave length of UV detector was 254 nm. The temperature of the colunm was 25℃, sample size was 10μL.RESULTS 93.2%～98.5% of synthetic colorants in the capsules were recovered, the peak concentrations were at the range of 5 ～ 60 mg·L^-1, Relative standard deviation was at the range of 0.8 % ～ 3.0%. CONCLUSION Different synthetic colorants are completely separated by the method.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2006年第4期303-305,共3页
Chinese Pharmaceutical Journal
