摘要
目的探讨小鼠皮内接种结核菌H37Ra后,腹腔MΦ是否被免疫激活以及激活后氮氧化物的产生、抗结核细胞因子的表达,从而了解H37Ra的免疫应答过程及对MΦ的抗菌免疫作用。方法用H37Ra、BCG皮内免疫小鼠后第30、60天,分别采用Griess法、化学法、ELISA法检测小鼠腹腔MΦ在受到、未受到IFN-γ刺激后NO、H2O2的产生以及IL-12、TNF-α的表达水平。结果①H37Ra免疫小鼠后能显著诱导MΦ分泌表达IL-12、TNF-α,与BCG皮内接种组、未免疫组比较,其差异均有统计学意义(P<0.05);也能诱导MΦ产生NO、H2O2,与未免疫组比较具有统计学意义(P<0.05),与BCG皮内接种组比较,无明显差异;②IFN-γ对巨噬细胞氮氧化物的产生、细胞因子的表达具增强效应。结论H37Ra皮内接种小鼠后能诱导巨噬细胞活化并产生较强的激活效应,且该效应略优于BCG。
To determine whether the celiac macrophages are activated and the production of nitric oxide and expression of the anti-tuberculous cytokines after macrophage activation, male C57BL/6 mice were immunized intracutaneously with Mycobacterium tuberculosis H37Ra, and the production of NO and H2O2 as well as the expression levels of IL-12 and TNF-α of the IFN-γ-stimulated or un-stimulated mouse celiac macrophages were determined by Griess＇ s method, chemical method and ELISA assay respectively. Thirty and 60 days after the intracutaneous vaccination, it was found that immunization of M. tuberculosis H37Ra strain could induce macrophages effectively to secrete IL-12 and TNF-α with sigificant differences with the BCG-intracutaneously immunized group and the un-immunized group. In addition, it also induce macrophages to produce NO and H2O2 with significant difference to the un-immu- nized group , but without any difference with the BCG-immunized group of mice. IFN-γ demonstrated an intensive effect on the production of nitric oxide and cytokines from macrophages. It is concluded that the intracutaneous vaccination with M. tuberculosis H37Ra strain can induce activation of macrophages and generate strong immune responses. This effect may be better than that of immunization with BCG .
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2006年第3期232-235,共4页
Chinese Journal of Zoonoses
基金
重庆市教委(2004)第12号科研基金
重庆医科大学科技创新基金(CX200204)资助项目
