摘要
目的探讨细胞因子TNF-α,IL1β,IL6在慢性乙型重型肝炎中与病毒含量的关系及其在重型肝炎中的作用和临床意义。方法60例慢性乙型重型肝炎中的血清标本进行HBVDNA和TNF-α,IL1β,IL6的测定,方法分别采用荧光定量PCR和ELISA法,并进行临床观察。结果慢性乙型重型肝炎中病毒载量不同组之间TNF-α,IL1β,IL6有显著性区别P<0.05病毒载量高时TNF-α活性值较高而IL1,βIL6则表现活性值低;另存活组病人TNF-α低,IL1,IL6升高较死亡组,P<0.05。结论慢性乙型重型肝炎中病毒含量一定程度上影响着细胞因子的变化,影响病情预后,对临床有一定的指导意义。
Objective To study the relationship between the loads of virus HBV DNA and TNF-α,IL1β,IL6 in patients with chronic gravis of hepatitis B. Methods The activities in the sera of 60 patients with chronic gravis of hepatitis B were detectd by ELISA and HBVDNA by PCR. Results The TNF-α,IL1β,IL6 activities were significantly changed, depending on the differences of the loads of virus HBVDNA（P〈0. 05）. The IL1β and IL6 activities in cured patients were higher than those in the death and TNF-α activities in cured patients were lower than those in the death P〈0. 05. Conclusion the loads of virus HBVDNA in patients with chronic gravis of hepatitis B influence the activities of cytokine TNF-α,IL1β,IL6. It is value in the to predict patient effect of cruing.
出处
《江西医学院学报》
CAS
2006年第1期16-18,共3页
Acta Academiae Medicinae Jiangxi
基金
江西省卫生厅课题资助项目(No.G010520)
