摘要
目的探讨无锡地区慢性乙型重型肝炎HBV基因型和核苷酸多态性分布的特点。方法套式PCR扩增HBVDNAS基因后作序列分析。结果50例慢性乙型重型肝炎患者B基因型1例(2%),C基因型43例(86%),BC混合基因型6例(12%);各基因型死亡率无显著差异,P>0.05。B、BC混合基因型S基因第139位、140位、146位、167位、170位、176位、191位、248位、254位、328位、337位、377位核苷酸与C基因型S基因第145位、157位、203位核苷酸各有聚集性改变,其中139与140位、145与146位有联合改变,并导致相应氨基酸的置换。结论无锡地区慢性乙型重型肝炎多见于C、BC混合基因型,C、B、BC基因型核苷酸呈多态性改变。
Objective To explore the features of genotypes and nucleotide polymorphisms in the patients with severe chronic hepatitis B in Wuxi region. Methods HBV DNA was amplified by nested polymerase chain reaction, and S gene fragment was detected by direct sequencing. Results The results of genotyping showed that among 50 patients with severe chronic hepatitis B 1 case （ 2% ） was genotype B,43 cases（86% ） were genotype C, 6 cases （12%） were mixed genotypes （B and C）. The mortality of genotype C and mixed genotypes were 39.5% （17/43） and 50% （3/6） respectively. There was no significant difference between genotype B/mixed B, C genotypes and genotype C for mortality （ X^2 = 0. 21, P 〉 0. 05 ）. The assembling mutation in S gene, which caused amino acid substitutions, occurred at the nucleotide site 139,140,146,167,170,176,191,248,254,328,337 and 377 in genotype B and mixed genotype B and C,and at nucleotide site 145,157 and 203 in genotype C. The mutations Of points 139/140 and 145/146 were bound together. The variations of nucleotides between genotype B/B, C and genotype C were different. Conclusions The results suggest that the patients with severe chronic hepatitis B may related to both genotype C and mixed genotypes B and C, and all the three genotypes presented nucleotide polymorphisms.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2005年第5期339-340,共2页
Chinese Journal of Clinical Laboratory Science
基金
江苏省科委基金项目(BS2003615)
无锡市自然科学基金资助(CK030013)。
