摘要
目的观察不同强度恒磁场对糖基化终产物作用下人脐静脉内皮细胞一氧化氮生成及一氧化氮合酶活性的影响。方法采用体外培养第3代人脐静脉内皮细胞,糖孵育法制备糖基化终产物修饰牛血清白蛋白。实验分为6组,即对照组、糖基化终产物修饰牛血清白蛋白(100μg/L)组及糖基化终产物修饰牛血清白蛋白+不同强度(1×10-4T、5×10-4T、10×10-4T2、0×10-4T)磁场组。各组细胞于培养及磁场作用24 h后收集标本,用Griess法检测培养上清中一氧化氮水平,并测定人脐静脉内皮细胞的一氧化氮合酶活性。结果糖基化终产物修饰牛血清白蛋白组一氧化氮含量和一氧化氮合酶活性较对照组明显降低(P<0.05)。1×10-4T、5×10-4T、10×10-4T和20×10-4T恒磁场组一氧化氮生成、一氧化氮合酶活性显著高于糖基化终产物修饰牛血清白蛋白组(P<0.05)。结论糖基化终产物修饰牛血清白蛋白可抑制人脐静脉内皮细胞的一氧化氮合酶活性和一氧化氮产生;1×10-4T^20×10-4T的恒磁场可呈强度依赖性拮抗糖基化终产物修饰牛血清白蛋白对一氧化氮生成的抑制效应。
Aim To investigate effects of constant magnetic field （CMF） on nitric oxide （NO） production and nitric oxide synthase （NOS） activity in human umbilical vein endothelial ceils （hUVEC） intervened by advanced glyeosylation endproduet （AGE）. Methods The third passage of cultured hUVEC were used. AGE modified bovine serum albumin （AGE-BSA） was prepared by sugar incubation. The cells were divided into six groups： control group, AGE group （AGE-BSA 100 μg/L）, AGE-BSA with 1×10^-4T, 5×10^-4T, 10 ×10^-4 T and 20 ×10^-4T of cons tant magnetic field group . Samples were collected 24 h later. NO levels in the supematant were determined using Griess reagent, and NOS activity of hUVEC was dedected. Results NO levels and NOS activity significantly decreased in AGE-BSA group（P〈0.05）. In 1×10^-4T, 5×10^-4T, 10×10^-4T and 20×10^-4 T groups, NO levels and NOS activity significantly increased compared with AGE-BSA group （ P 〈 0.05）. Conclusion AGE- BSA can inhibit NO production and NOS activity in hUVEC, whereas 1 ×10^-4 T to 20 ×10^-4 T of constant magnetic field can intensity-dependently antagonize the effects of AGE-BSA on NO production.
出处
《中国动脉硬化杂志》
CAS
CSCD
2005年第5期537-540,共4页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金(30270396)资助
