摘要
目的探讨内源性胍丁胺对氧化低密度脂蛋白(OXIDIZED LOW DENSITY LIPOPROTEIN,OX-LDL)诱导的单核-内皮细胞黏附的作用及其可能的机制。方法体外培养人脐静脉内皮细胞(HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS,HUVEC),用不同浓度的胍丁胺作用于OX-LDL诱导的HUVEC24H,加或不加硝基精氨酸甲酯(L-NAME),观察人单核细胞对HUVEC的黏附及ICAM-1在HUVEC的蛋白表达。结果胍丁胺浓度依赖地抑制OX-LDL诱导的单核细胞对HUVEC的黏附及ICAM-1在HUVEC的表达(P<0.01),二者成正相关(R=0.8857,P<0.001),加入L-NAME后AGM的作用减弱(P<0.05)。结论AGM可能通过下调ICAM-1的表达抑制循环单核细胞与内皮细胞的黏附,该作用与一氧化氮合酶(NOS)有关。
Objective To investigate the effect of endogenous agmatine on monocyte/endothelial cell adhesion induced by oxidized low density lipoprotein （ox-LDL） and to elucidate the mechanism. Methods Human umbilical vein endothelial cells （HUVECs） were cultured and identified, then divided into four groups： AGM group in which the HUVECs were cultured in serum-free culture fluid containing 10^-8, 10^-7 10^-6, 10^-5, 10^-4, 10^-3 moL/L AGM for 20 h, then with addition of 100 mg/L ox-LDL for 4 h; L-NAME ＋ AGM group in which only NG-Nitro-L-Arginine Methyl Ester （L-NAME）mNOS depressor was added 30 min before the process of AGM group ; ox-LDL group in which the HUVECs were cultured in serum-free culture fluid for 20 h, then with the addition of ox-LDL for 4 h ; control group in which the HUVECs were cultured only in serumfree culture fluid. Monocytes were isolated from peripheral blood. Then the ICAM-1 expression was detected by immunocytochemistry and monocytes adhesion was detected. Results Agmatine significantly inhibited the adhesion of monocytes to HUVECs and the expression of ICAM-1 induced by ox-LDL in a dose dependent manner （P 〈0. 01 ）. The ICAM-1 expression was positively correlated with the monocytes adhesion （r =0. 885 7, P 〈 0. 001 ）, and this effect was attenuated by L-NAME （P 〈 0. 05 ）. Conclusion AGM is a potent in vivo inhibitor of monocyte/endothelial cells adhesion. The effect could be ICAM-1-dependent and correlated with nitric oxide synthase.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第4期334-337,共4页
Journal of Third Military Medical University
