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胃癌细胞体外药敏试验中的条件优化

A Feasible Study For Chemosensitivity of Gastric Cancer in Vitro
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摘要 目的:探讨胃癌细胞体外药敏试验中的适宜条件。方法:利用正交试验,取3个水平4个因素进行全面考察,筛选出最佳实验条件。结果:胃癌细胞体外药敏试验中应取(2~3)×10^4个/ml细胞密度,并在接种后立即加入10PPC的抗癌药物,药物作用时间为72h。结论:经正交试验筛选出的最佳试验条件可有效提高体外药敏试验的灵敏度,为临床个体化疗提供方法学依据。 Objective: To inverstigate the best condition in chemosensitivity of gastric cancer cell line-SGC in vitro. Methods: Design orthogonal test to screen the best condition of drug sensitivity test. Resuits: The best condition was that the number of seeded cell was (2 - 3 ) × 10^4/ml ,the drug concentration was 10PPC and the killing time was 72 h. Conclusion: The best condition can improve the sensitivity for drug sensitivity test, and maybe useful for individual chemotherapy.
出处 《江苏大学学报(医学版)》 CAS 2006年第1期41-43,T0001,共4页 Journal of Jiangsu University:Medicine Edition
关键词 正交试验 SGC胃癌细胞株 药敏试验 Orthogonal test Gastric cancer cell line-SGC Drug sensitivity test
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  • 1辛华雯,王润帮,杜光祖,林鲁杰.十种常用抗癌药体外敏感性与临床疗效相关性研究[J].中国医院药学杂志,1994,14(12):548-550. 被引量:29
  • 2[1]Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays[J]. J Immunol Methods,1983,65(1-2):55-63. 被引量:1
  • 3[2]Taylor CG, Sargent JM, Elgie AW, et al. The clinical relevance of chemosensitivity testing in ovarian cancer[J]. Cancer Detect Prey,1998,22(4) :305-312. 被引量:1
  • 4[3]Sargent JM. The use of the MTT assay to study drug resistance in fresh tumour samples[J]. Recent Results Cancer Res, 2003,161(1) :13-25. 被引量:1
  • 5[4]Fujita K, Kubota T, Matsuzaki SW, et al. Further evidence for the value of the chemosensitivity test in deciding appropriate chemotherapy for advanced gastric cancer[J]. Anticancer Res, 1998,18(3B) :1973-1978. 被引量:1
  • 6[5]Kubota T, Egawa T, Otani Y, et al. Cancer chemotherapy chemosensitivity testing is useful in evaluating the appropriate adjuvant cancer chemotherapy for stages Ⅲ/Ⅳ gastric cancers without peritoneal dissemination[J]. Anticancer Res, 2003,23(1B):583-587. 被引量:1
  • 7[6]Tanino H, Oura S, Hoffman RM, et al. Acquisition of multidrug resistance in recurrent breast cancer demonstrated by the histoculture drug response assay[J]. Anticancer Res, 2001,21 (6A) :4083-4086. 被引量:1
  • 8李振,恶性肿瘤化学治疗学,1990年 被引量:1
  • 9J.K wilson,J.M.Sargent,A.W.Elgie,et al,A feasibility.study of the MTT assay for chemosensitivity testing in ovarian malignancy. British Journal of Cancer . 1990 被引量:1
  • 10Toshibaru Furakawa MD,Testuto Kubota MD,Akihiko Suto MD et al.Clinical usefulness.of chemosensitivity testing using the MTT assay. Journal of Surgery . 1992 被引量:1

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