摘要
目的:探讨胃癌细胞体外药敏试验中的适宜条件。方法:利用正交试验,取3个水平4个因素进行全面考察,筛选出最佳实验条件。结果:胃癌细胞体外药敏试验中应取(2~3)×10^4个/ml细胞密度,并在接种后立即加入10PPC的抗癌药物,药物作用时间为72h。结论:经正交试验筛选出的最佳试验条件可有效提高体外药敏试验的灵敏度,为临床个体化疗提供方法学依据。
Objective: To inverstigate the best condition in chemosensitivity of gastric cancer cell line-SGC in vitro. Methods: Design orthogonal test to screen the best condition of drug sensitivity test. Resuits: The best condition was that the number of seeded cell was (2 - 3 ) × 10^4/ml ,the drug concentration was 10PPC and the killing time was 72 h. Conclusion: The best condition can improve the sensitivity for drug sensitivity test, and maybe useful for individual chemotherapy.
出处
《江苏大学学报(医学版)》
CAS
2006年第1期41-43,T0001,共4页
Journal of Jiangsu University:Medicine Edition
关键词
正交试验
SGC胃癌细胞株
药敏试验
Orthogonal test
Gastric cancer cell line-SGC
Drug sensitivity test