摘要
目的研究大鼠脑出血后血肿周围水通道蛋白4(AQP4)的动态变化及其与脑水肿、脑损伤程度的关系。方法采用SD大鼠自体动脉血注入尾状核建立脑出血模型,应用免疫组织化学法、干湿重法、Alexis法分别检测大鼠脑出血后不同时间点血肿周围AQP4、脑组织含水量及神经功能缺损程度的动态变化。结果①血肿周围AQP4在脑出血后12h表达开始增强,1～3d达到高峰,7d后略高于正常,14d基本恢复正常。②脑组织含水量在出血后6h增加,1～3d达到峰值,7d明显减轻,14d基本恢复正常。③神经功能缺损从出血后6h开始,最重的时间点是12h～3d,7d明显减轻,14d基本恢复正常。④脑出血后血肿周围AQP4的表达与脑组织含水量之间存在关联(x2mh=16.49,P<0.05)。⑤脑出血后血肿周围AQP4的表达与神经功能缺损程度之间存在关联(x2mh=15.07,P<0.05)。结论①AQP4的表达与脑水肿、脑损伤的发生发展密切相关,是引起出血性脑水肿脑损伤的重要因素之一。②早期积极控制脑出血后AQP4的过度表达将是减轻和预防脑出血后脑水肿脑损伤的有效途径。
Objective This paper aimed to explore the correlation of aquaporin-4 （AQP-4） expression with cerebral edema and injury, by studying the dynamic changes of AQP-4 expression around hematoma in rats following intracerebral hemorrhage （ICH） . Methods The experimental ICH model was established in SD rats by injecting the rats＇ own artery blood into the caudate nucleus. Immunohistochemistry straining was used to detect the AQP-4 expression around hematoma at 6 and 12 hrs and 1, 3, 7 and 14 days after ICH. The brain water content was measured by the wet/dry weight method. Alexis method was used to examine the neurological deficit scores. Results The AQP-4 expression around hematoma increased at 12 hrs and reached a peak between 1-3 days after ICH. It remained higher than normal level at 7 days and returned to normal at 14 days basically following ICH. The brain water content increased from 6 hrs and peaked between 1-3 days following ICH. It was obviously reduced at 7 days and returned to normal at 14 days following ICH. The neurological deficit appeared from 6 hrs and the severest injury was observed between 12 h-3 d after ICH. The deficit was obviously assented at 7 days and recovered at 14 days after ICH. The expression level of AQP-4 around hematoma was positively correlated with the brain water content （2mh^2 = 16.49, P 〈 0.05 ） and neurological deficit scores （Xmh^2 = 15. 07, P 〈 0.05 ）. Conclusions AQP-4 may play a role in the development of hemorrhagic cerebral edema and injury after ICH. The hemorrhagic cerebral edema and injury after ICH can be alleviated by inhibiting the AQP-4 expression.
出处
《国际神经病学神经外科学杂志》
2006年第1期5-8,共4页
Journal of International Neurology and Neurosurgery
关键词
水通道蛋白-4
脑出血
脑水肿
aquaporin-4
intracerebral hemorrhage
brain edema
