摘要
目的:探讨神经生长因子(NGF)和其受体酪氨酸激酶受体A(trkA)和总神经营养因子受体(p75)变化对哮喘大鼠气道炎症的影响。方法:通过鸡卵蛋白致敏激发建立哮喘模型,并将32只SD大鼠随机分为4组:正常对照组、哮喘组、外源性NGF干预组(NGF组)及抗NGF抗体干预组(抗NGF组)。各组测定支气管肺泡灌洗液(BALF)总细胞及细胞分类计数,并行支气管肺组织切片HE染色观察病理改变;通过逆转录-聚合酶链反应(RT-PCR)半定量检测哮喘组和对照组肺组织NGFmRNA,4组肺组织trkA和p75mRNA表达变化。结果:哮喘组与对照组比较,BALF总细胞计数、嗜酸性粒细胞(Eos)计数及淋巴细胞(Lym)计数显著增多(均P<0.001),支气管肺组织炎症表现显著;肺组织NGFmRNA及trkA和p75mRNA表达明显增强(均P<0.01);哮喘组NGFmRNA与BALF细胞总数、Lym数呈正相关(均P<0.001)。NGF组与哮喘组比较,BALF总细胞、Eos及Lym计数均显著增多(均P<0.01),支气管肺组织炎症表现更为显著,p75和trkAmRNA表达明显增强(均P<0.05)。抗NGF组与哮喘组比较,BALF总细胞、Eos及Lym计数显著减少(均P<0.001),支气管肺组织炎症表现显著减轻,p75和trkAmRNA表达明显减弱(均P<0.01)。结论:致敏哮喘大鼠肺组织内NGFmRNA表达水平显著增高,并与气道炎症密切相关;NGF上调trkA和p75mRNA表达,从而增强NGF的功能效应,共同参与哮喘的气道神经源性炎症。
Objective To determine the expression of nerve growth factor （ NGF）, tyrosine kinase receptor A （trkA）, and pan-neurotrophin receptor （p75） in the lung tissues in asthmatic rats , and to explore their effects on the airway inflammation. Methods Thirty-two SD rats were randomly divided into 4 groups： the control, asthma, NGF and anti-NGF groups. The asthmatic model was established by the inhalation and injection of ovalbumin. The total cell count and differential cell count in the bronchoalveo- lar lavage fluid （BALF） were performed. The pathologic changes in the lung tissues of the 4 groups was detected by HE staining. The NGF mRNA expression in the lung tissues of the asthma and control groups was determined by reverse transcription-polymerase chain reaction （RT-PCR）. The changes of trkA and p75 mRNA expressions in the lung tissues in the 4 groups were also investigated by RT-PCR. Results Compared with the control group, the BALF total cell, the BALF eosinophils （Eos）, and the BALF lymphocytes （Lyms） significantly increased （All P 〈0. 001 ） in the asthma group; and the lung tissues of the asthma group had more infiltrating inflammatory cells. Not only the expression of NGF mRNA, but al-so its receptors trkA and p75 mRNA in the lung tissues were significantly higher in the asthma group than those in the control group （All P 〈0.01 ）. Positive correlation was found between the expression of NGF mRNA and the BALF total cell, the BALF Lyms in the asthma group. Compared with the asthma group, the total cell, the Eos, and the lyms in BALF in the NGF group significantly increased （All P 〈0.01 ） , and the lungs of the NGF group had apparent inflammatory changes. The expressions of p75 and trkA mRNA were enhanced significantly （All P 〈 0.05 ）. Compared with the asthma group, the total cell,the Eos, and the lyms in BALF in the anti-NGF group significantly decreased （All P 〈 0. 001 ）, and the lungs of the anti-NGF group showed alleviative inflammatory changes. The
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2005年第6期660-665,共6页
Journal of Central South University ：Medical Science
基金
湖南省卫生厅科研基金(B2003-035)
