摘要
目的:初步探讨支气管哮喘Th1/Th2类细胞因子免疫失衡对脾淋巴细胞神经生长因子(NGF)mRNA表达的影响。方法:采用鸡清卵蛋白建立SD大鼠哮喘模型,分离脾淋巴细胞并培养,通过逆转录-聚合酶链反应(RT-PCR)半定量法测定细胞在刀豆素A(ConA)刺激下表达NGFmRNA的基础水平,并观察予γ-干扰素(IFN-γ)和白细胞介素-4(IL-4)干预后NGFmRNA表达的改变。结果哮喘大鼠脾淋巴细胞在基础状态下具有表达NGFmRNA的功能,并在ConA刺激下呈时间依赖性增强。50μg/LIL-4干预12h、24h、36h及48h时NGFmRNA相对表达量随时间延长逐渐增高,并分别与同时间点的基础值比较有统计学意义(均P<0.01);0,10,50,100μg/L的IL-4干预24h时NGFmRNA表达量依次增高,分别比前一梯度低浓度干预组有显著增高(P<0.05)。10μg/LIFN-γ干预12h、24h、36h、48h时NGFmRNA相对表达量分别与同时间点的基础值相比有显著下降(均P<0.01);0,1,10,50μg/L的IFN-γ干预24h时NGFmRNA表达量依次减低,分别比前一梯度低浓度干预组有显著降低(P<0.05)。结论:在哮喘中Th2类细胞因子IL-4可上调淋巴细胞NGFmRNA表达,Th1类细胞因子IFN-γ可下调淋巴细胞NGFmRNA表达,两者均呈时间和浓度依赖性;Th1/Th2类细胞因子免疫失衡可能通过调控NGFmRNA表达间接促进其诱导神经源性炎症。
Objective To explore the effect of Th1/Th2 cytokines on the expression of nerve growth factor （NGF）in splenic lymphocytes in asthmatic model. Methods Four SD rats were sensitized and challenged with ovalbumin to establish an asthmatic model, and the rat splenic lymphocytes were isolated and cultured with ConA. The expressions of NGF mRNA were detected by reverse transcription-polymerase chain reaction （RT-PCR） , and were observed after the lymphocytes were exogenously added with interferon-γ（IFN-γ） or interleukin-4 （ IL-4 ）. Results The lymphocytes of the asthmatic model stimulated by ConA in vitro expressed NGF mRNA in a time-dependent manner. After the lymphocytes had been cultured with IL-4 for 12 h, 24 h, 36 h, and 48 h, 50 μg/L IL-4 upregulated the expressions of NGF mRNA in a time -dependent manner and all the NGF mRNA expressions were significantly higher than the basal values at the same time （ all P, 〈 0.01 ）. After 0, 10, 50, and 100 μg/L IL-4 had been added for 24 h, IL-4 upregulated the expressions of NGF mRNA in a dose-dependent manner and the NGF mRNA expressions were all significantly higher than the values of the lower dose IL-4 （ all P, 〈 0.05 ）. After the lymphocytes had been cultured with 10 μg/L IFN-γ for 0 h, 12 h, 24 h, 36 h, and 48 h, IFN-γ downregulated the expressions of NGF mRNA in a time-dependent manner and all the NGF mRNA expressions were significantly lower than the basal values at the same time（ all P, 〈0.01 ）. After 0, 1 , 10, and 50 μg/L IFN-γ have been added for 24 h, IFN-γ downregulated the expressions of NGF mRNA in a dose-dependent manner and all the NGF mRNA expressions were significantly lower than the values of the lower IFN-γ dose （ all P 〈 0.05 ）. Conclusion In the splenic lymphocytes of asthmatic rats, IL-4, one of the ThE cytokines, can upregulate the expressions of NGF ; IFN-γ one of the Th1 cytokines, can downregulate the expressions of NGF both in a time-dependent manner and in a dose-dependent manner. Th1/Th2 cyto
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2007年第1期119-123,共5页
Journal of Central South University ：Medical Science
基金
湖南省卫生厅科研基金~~
