摘要
建立了一种高效液相色谱法测定百菌清有效成分含量的新方法。采用250×4．0mm Lichosorb Si60-5μ色谱柱,以正庚烷-二氯甲烷(80:20V/V)N流动相,流速为1．2ml/min,254nm紫外检测器。该 方法处理简单、快速、准确、适用,方法线性范围为0．03-1．2mg/ml,回收率为99．01％-100．72％,变异系 数为0．277％。
The new method for the 4.0ram Lichosorb Si60- 51μchroma determination of active ingredient in chlorothalonil by HPLC on 250x tographic column with 254nm UV-detector was established. The mobil phase was n-heptane - methylene chloride （80：20WV） and flow rate was set at 1.2ml/min,The method was simple, rapid, accurate and practical. The linear correlation were 0.03 -1.2mg/ml, the recovery were 99.01%-100.72%, the coefficients of variation were 0.277%.
出处
《中国农学通报》
CSCD
2005年第12期352-353,共2页
Chinese Agricultural Science Bulletin
