摘要
通过除虫菊组织培养技术的正交试验及优化筛选,建立了除虫菊最适繁殖培养基:MS+BA0.3 mg/L+NAA 0.2mg/L;通过在培养基中添加不同浓度生长素,得到适合除虫菊的生根培养基:1/2MS+IAA 0.2 mg/L+ABT 0.1 mg/L。建立了除虫菊根尖染色体鉴定的最佳条件。染色体鉴定结果表明:白花除虫菊的染色体为2n=18。为除虫菊的种质保存和优良品种的选育工作奠定了基础。
The fittest media of Pyrethrum cinerariifolium for rapid-propagation was established and optimized by orthogonal test in tissue culture process. The obtained resuls indicated that the fittest rapidpropagation media of Pyrethrum cinerariifolium is MS＋BA 0. 3 mg/L＋NAA 0. 2 mg/L and its rooting media is 1/2MS＋IAA 0. 2 mg/L＋ABT 0. 1 mg/L. The best condition of chromosome determination was established. The chromosomal number of pyrethrum was 2n=18. The above research laid the basis for preserving the plant resource and breeding excellent lines of Pyrethrum cinerariifolium.
出处
《药物生物技术》
CAS
CSCD
2005年第6期370-374,共5页
Pharmaceutical Biotechnology
关键词
白花除虫菊
组织培养
染色体鉴定
Pyrethrum cinerariifolium, Tissue culture, Identification of chromosome
