摘要
采用沙土鼠20只,随机分为两组,夹闭双侧颈总动脉致前脑缺血后再灌流性脑损伤。两组均于右侧大脑皮质体感区注入2.5％HRP20μl。实验组动物腹腔注射蚓激酶10μl,而对照组动物腹腔注射生理盐水10μl。心脏灌流固定,取材、冰冻切片,行TMB法呈色反应,光镜下观察。结果表明:实验组10例126张切片中丘脑标记细胞总数为1885个,平均14.96个。对照组10例中126张切片中丘脑内标记细胞总数为502个,平均为3.99个。两组均为丘脑外侧核群标记细胞数量最多。
Twenty gerbils were used in this experiment ,they were divided into two groups by random sam-pling. We pressed the bilataral carotid arteries form both side with the arterial clips,after thirty min-utes, removed them,so,We gained the models ofthe injury that anterior cerebrums were reperfused after the Then,BET 10?1 was injected into the cavitas abdominis in the test group and salt solution 10v1 was used in the same place in the contrast group. HRP 20?1 was injected into the ca?itas abdominis of every-one at the area 3.2.1 of Brodmann.
The sections of thalamus ,though TMB dyeing,were studied under the light microscope,the result showed that:in the test group,the sum tatal of the marked cells in one humdred and twenty-six films was one thousand eight hundred and eight-five,averagt fourteen point ninety-six,inthe contrast group,the marked cells in one hunded and twenty-six films,amount to five hundred zero two,average three point ninety-nine the marked cells were both fonnd mainly at the lalteral nuclear group of thala-mus in two groups.
出处
《局解手术学杂志》
1996年第3期8-11,共4页
Journal of Regional Anatomy and Operative Surgery
