摘要
建立了伪狂犬病病毒的PCR检测方法,并将沪株伪狂犬病病毒的gE基因扩增片段进行测序和比较分析。根据伪狂犬病病毒gE和gB基因序列设计两对引物,以伪狂犬病病毒SH株DNA和单纯疱疹病毒I型为模板,产物连接到pMD18-T Vector。重组质粒测序后与闽A株序列比较,同源性为98%,与Genebank中已登录的伪狂犬病病毒gE基因序列进行blast比较,同源性在97%以上。gE基因序列比较保守,在疫苗应用及分子诊断方面均有较大的价值。
The PCR assay of Pseudorabies Virus （PRV） was established, and the amplified fragment of gE gene of PRV SH strain was sequenced and analyzed. Two pairs of PCR primers were designed according to the gE and gB genes of PRV. The PRV SH strain and Herpes simplex virus strain I were used as a template and the PCR product was cloned into pMD18-T vector. The recombinant plasmid was sequenced and compared with the Fa strain, and their homology was 98 %. In comparison with the gE gene sequence of PRV recorded in the Genebank, the homology was above 97%. The sequence of gE gene is comparatively conserved and has a rather high value in vaccine application and molecular diagnosis,
出处
《上海农业学报》
CSCD
2005年第4期5-8,共4页
Acta Agriculturae Shanghai
基金
上海市科技发展基金资助项目(024909006)
