摘要
目的:探讨腺苷A1受体拮抗剂对地氟醚短暂预处理脑保护作用的影响。方法:实验于2003-01/03在第四军医大学西京医院麻醉科实验室和放射科核磁共振室完成。36只SD大鼠,随机分为6组(n=6):生理盐水+地氟醚组,在地氟醚预处理(1个最低肺泡有效浓度,60m in)前30m in经腹腔注射生理盐水2.5m L/kg;二甲基亚砜+地氟醚组,在地氟醚预处理前30m in经腹腔注射二甲基亚砜2.5mL/kg;腺苷A1受体拮抗剂+地氟醚组,在地氟醚预处理前30m in经腹腔注射腺苷A1受体拮抗剂1m g/kg;生理盐水+O2组,在吸氧预处理前30m in经腹腔注射生理盐水2.5mL/kg;二甲基亚砜+O2组,在吸氧预处理前30m in经腹腔注射二甲基亚砜2.5m L/kg;腺苷A1受体拮抗剂+O2组,在吸氧预处理前30m in经腹腔注射腺苷A1受体拮抗剂1m g/kg。预处理结束1h后所有动物均采用右侧颈动脉丝线栓塞大脑中动脉致局灶性脑缺血120min,观察再灌注后24h动物神经系统改变及脑梗死范围。结果:36只大鼠脑缺血再灌注后24h均存活,均进入结果分析。①预处理期间各组生理参数比较:直肠温度、动脉血氧分压、动脉血二氧化碳分压、血压及血糖在预处理期间各组间无明显差异。②神经行为学改变:腺苷A1受体拮抗剂+地氟醚组再灌后24h神经行为学评分为(3.00±0.89)分,明显高于生理盐水+地氟醚组和二甲基亚砜+地氟醚组,与吸氧预处理各组无明显差异。③脑梗死范围:弥散加权法所测为(247.9±38.24)mm3,三苯四氯氮唑染色法所测为(301.70±41.47)m m3,均明显高于生理盐水+地氟醚组和二甲基亚砜+地氟醚组,与吸氧预处理各组无明显差异;吸氧预处理三组间也无显著性差异。结论:腺苷A1受体阻滞剂8-环戊基-1,3-二丙基黄嘌呤可消除短暂地氟醚预处理产生的脑保护作用,表明该作用可能与腺苷A1受体相关。
AIM： To investigate the effects of adenosine A1 receptor antagonist on the cerebral protective action of desflurane short pretreatment. METHODS： The experiment was done in the laboratory of Department of Anesthesiology and Room of Nuclear Magnetic Resonance, Department of Radiology, Xijing Hospital, Fourth Military Medical University of Chinese PLA from January to March 2003. Thirty-six Sprague-Dawley rats were randomly assigned into six groups （n=6）： In saline ＋ desflurane group, the rats were treated with 2.5 mL/kg saline through intraperitoneal injection at 30 minutes before the desflurane pretreatment （one minimum alveolar concentration, 60 minutes）; In dimethyl sulfoxide ＋ desflurane group, the rats were treated with 2.5 mL/kg dimethyl sulfoxide through intraperitoneal injection at 30 minutes before the desflurane pretreatment. In adenosine A1 receptor antagonist ＋ desflurane group, the rats were treated with 1 mg/kg adenosine A1 receptor antagonist through intraperitoneal injection at 30 minutes before the desflurane pretreatment. In saline＋O2 group, the rats were treated with 2.5 mL/kg saline through intraperitoneal injection at 30 minutes before the sucking oxygen pretreatment. In dimethyl sulfoxide ＋O2 group, the rats were treated with 2.5 mL/kg dimethyl sulfoxide through intraperitoneal injection at 30 minutes before the sucking oxygen pretreatment. In adenosine A1 receptor antagonist ＋O2 group, the rats were treated with 1 mg/kg adenosine A1 receptor antagonist through intraperitoneal injection at 30 minutes before the sucking oxygen pretreatment. One hour after the ending of pretreatment, all the animals were induced focal cerebral ischemia for 120 minutes with thread occlusion on middle cerebral artery of right-side carotid artery. The alteration of neural system and range of cerebral infarction at 24 hours after reperfusion in animals were observed. RESULTS： Thirty-six rats were living at 24 hours after cerebral ischemia/ reperfusion, and were all involved in the result a
出处
《中国临床康复》
CSCD
北大核心
2005年第42期102-104,i0003,共4页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金资助项目(30170907)~~
