摘要
目的 通过制作大鼠脑缺血模型和运用兴奋性氨基酸NMDA型受体阻滞剂MK 80 1,观察海马兴奋性氨基酸 (EAA)的变化及其所致细胞结构改变。方法 采用Pulsinelli氏改良方法 ,分别测定大鼠海马在脑缺血不同时期EAA的改变及MK 80 1对EAA所致结构变化的影响。结果 提示脑缺血时EAA明显升高 ,且缺血时间越长释放越多 ,经过MK 80 1治疗细胞损伤明显减少。结论 大鼠脑缺血后EAA明显升高 ;MK 80 1能阻止EAA对脑的损伤 。
Objective The model of ischemic rats was made and MK?801 was applied to observe the change of excitatory amino acid (EAA) and also pathological change in rat hippocampus. Methods The quantity of EAA at different ischemic time was measured by using the improving method of Pulsinelli's and the morphologic change in hippocampus after induced ischemia was studied with the application of MK?801. Results After induced ischemia, the EAA level raised significantly by the time and MK?801, a EAA receptor antagonist, could reduce the damage of cells in hippocampus. Conclusion EAA level increased with the degree and time of ischemia. MK?801 can prevent the damage from EAA in ischemia.
出处
《同济大学学报（医学版）》
CAS
2001年第1期25-27,共3页
Journal of Tongji University(Medical Science)
基金
铁道部科技基金课题 (J98Z0 16 )
