摘要
目的:探讨bc l-2和bax蛋白在人脑胶质瘤中的表达及其与恶性程度的关系。方法:对本院神经外科住院手术切除的脑胶质瘤组织标本60例用免疫组化法检测bc l-2和bax蛋白的表达,用TUNEL法检测细胞凋亡。结果:Ⅱ、Ⅲ、Ⅳ级胶质瘤间bc l-2、bax蛋白表达阳性细胞率分别为(50.55±1.63)%、(35.14±0.87)%、(15.42±1.56)%和(15.08±0.99)%、(28.15±1.58)%、(39.58±1.66)%(均P<0.05)和凋亡细胞百分率(4.58±1.05)%、(10.26±1.66)%、(19.78±1.45)%(P<0.05),差异有显著性。胶质瘤的分级与bc l-2蛋白阳性细胞率呈负相关(r=-0.8991,P<0.05),与bax蛋白表达和凋亡细胞百分率呈正相关(r=0.8856,0.9047,P<0.05)。结论:bc l-2、bax蛋白表达阳性细胞率和凋亡细胞百分率与胶质瘤的恶性程度有关。
Objective：To explore the bel-2 and bax expression in human gliomas and its relation with the degree of glioma malignancy. Methods： bcl-2 and bax protein expression positive cell rates in 60 human brain gliomas were determined by immunohistochemical technique, and apoptotic cell rates were evaluated by TUNEL method. Results： The differences of bcl-2 and bax protein expression positive cell rates respectively （50.55±1.63 ）% , （35. 14±0.87）%,（15.42±1.56）% and （15.08±0.99）%,（28. 15±1.58）%,（39.58±1.66）%,all（P〈0.05） and apoptotic cell rates （ 4. 58±1.05）%,（10.26±1.66）%, （19.78±1.45）%, （P 〈0.05） were statistically significant among Ⅱ, Ⅲ and Ⅳ malignant degrees in gliomas. The malignant degrees of gliomas were positively correlated with bax protein expression cell rate and apoptotic cell rate （ r = 0. 8856, 0. 9047, P 〈 0.05） , but was negatively correlated to bcl-2 protein expression cell rate （r = -0. 8991,P 〈 0.05）. Conclusions： Bcl-2 and bax protein positive cell rates and apoptotic cell rate could be related to the degrees of malignancy of human brain gliomas.
出处
《广州医学院学报》
2005年第4期21-23,共3页
Academic Journal of Guangzhou Medical College
