摘要
目的 研究抑癌基因Rb在人胶质瘤SHG 44细胞调亡前后的表达情况。方法 利用N端Rb蛋白单克隆抗体通过Western印迹法检测SHG 44胶质瘤细胞株Rb蛋白的表达情况 ,并观察用VP16诱导细胞凋亡后Rb蛋白表达的改变。结果 在SHG 44胶质瘤细胞株中存在一相对分子质量约 70× 10 3 、具有N端的Rb蛋白。细胞凋亡后 ,Rb蛋白有裂解现象 ,出现一具有N端的约 48× 10 3 的裂解蛋白 ,并使细胞停滞于G1期。结论 在胶质瘤SHG 44细胞中有Rb基因突变体的表达 ,Rb蛋白的裂解与细胞凋亡密切相关。
Purpose To study expression of the tumor suppressor gene retinoblastoma protein (Rb) before and after apoptosis of SHG?44 glioma cell. Methods Rb expression was detected in SHG?44 cells by Western blot using N terminal Rb monoclone antibody.And the change of Rb expression after apoptosis induced by VP16 was examined. Results A 70×10 3 with N terminal Rb was found in SHG?44 cell line.After cell apoptosis,Rb was cleaved into a 48×10 3 protein with N?terminal and the glioma cells were arrested at G 11 phase. Conclusions A mutant Rb protein was expressed in SHG?44 cells.There is close relationship between Rb cleavage and apoptosis in glioma.
出处
《上海医科大学学报》
CSCD
2000年第2期102-104,共3页
Journal of Fudan University(Medical Science)
