摘要
目的:探讨人基因重组骨形成蛋白-2(rhBMP-2)对牙周韧带细胞群(PDLC)成纤维表型的影响.方法:PDLC在含不同浓度的rhBMP-2的培养液中培养,采用免疫细胞化学技术做表皮生长因子受体(EGFR)的检测,并用图像分析软件分析EGFR表达的强弱.结果:rhBMP-2作用下,PDLC的EGFR的表达有变化(P<0.05),25～100 μg/L组EGFR的表达减弱,100 μg/L组最弱,随rhBMP-2浓度上升,EGFR的表达又有增强的趋势.结论:rhBMP-2对PDLC的成纤维表型产生了影响,合理剂量的rhBMP-2应用可以保证PDLC成纤维表型的较高表达,使种植体周牙周韧带的构建成为可能.
Objective： To explore the effects of rhBMP- 2 on the fibroblastic phenotype of PDLC. Methods： PDLC were incubated under different concentration of rhBMP - 2, the expression of EGFR was detected by immunocytochemistry technique, and then analyzed with HMIAS - 1000 color image system. Results： rhBMP -2 had an effect on the expression of EGFR（P〈O.05） , which decreased in the range of 25μg/l - 100μg/l rhBMP -2 and had the lowest expression at 100μg/ 1 rhBMP - 2, but up - regulated as the concentration of rhBMP - 2 increased. Conclusions： rhBMP - 2 has an effect on the fibroblastic phenotype of PDLC, and the appropriate dose of rhBMP - 2 can assure the stability of the fibroblastic phenotype , which makes it possible to construct periodontal ligament around dental implant.
出处
《口腔医学研究》
CAS
CSCD
2005年第4期375-377,共3页
Journal of Oral Science Research
基金
国家自然科学基金项目(编号:30171021)
