摘要
利用聚乙烯醇（ＰＶＡ）包埋法共固定糖化酶和葡萄氧化酶（ＧＯＤ）制成酶膜，与氧电极结合成为复合酶电极；该电极可以用于流动注射分析系统中测定溶液中麦芽糖。对酶电极的ｐＨ效应和温度效应作了研究。酶电极的线性范围为０．５－３５ｍｍｏｌ／Ｌ．响应周期小于２ｍｉｎ。变异系数（ＣＶ）为１．８％。在半连续使用状态下，酶电极可以使用１０ｄ以上。糟化酶保持活力为６０％。对延长酶电极寿命和克服共存葡萄糖的干扰的方法作了探讨。
Amyloglucosdase was coentrappedwith glucose oxidase(GOD)in a poly vinyl al-cohol membrane.The multi-enzyme mem-brane was combined with an oxygen electrodeto form a bienzyme electrode,which could beincorporated in a flow injection system to de-termine maltose,Temperature effect and pHeffect on the bienzyme electrode system wereinvestigated.The linear respones range forthe multi-enzyme electrode was 0.5-35mmol/L of sugar.The entire response cyclewas less than 2min,The variation coeffect(CV)was 1.8%。The bienzyme electrode wasused semi-continuously for 10 days,at the endof which period its respones to maltosere-mained 60%of its initial value,Methods toextend the life time of the bienzyme electrodeand to eliminate the interference of coexistingglucose are discussed.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1995年第1期72-76,共5页
Progress In Biochemistry and Biophysics
基金
国家"八五"攻关生物技术项目
关键词
麦芽糖
测定
麦芽糖传感器
复合酶电极
maltose determination, maltosesensor,poly vinyl alcohol(PVA),multi-en-zyme electrode,flow injection analysis(FIA)
