摘要
采用ＣＡＳ２００细胞成象分析仪，免疫组织化学，形态学测量以及组织病理学技术，对食管癌高发区人群食管上皮固有膜血管乳头增生状况（乳头的数目和高度增加），乳头区和非乳头区细胞的增生细胞核抗原（ＰＣＮＡ）和Ｐ５３肿瘤抑制基因蛋白聚集，以及上皮内嗜酸性粒细胞浸润在食管癌变中的意义进行初步研究。结果表明：随着食管上皮从正常到增生和间变的进行性发展，食管固有膜血管乳头数目增多，乳头高度增加，乳头区细胞的ＰＣＮＡ表达升高。轻度增生的病变，Ｐ５３蛋白聚集多发生在乳头区细胞；嗜酸性粒细胞的分布也与病变的进展程度有关。结果提示乳头区增生细胞可能是食管上皮最早发生癌变的部位；嗜酸性粒细胞浸润可能是机体对细胞增生的一种保护反应。
The primary study was performed on papillae and papillary-region cell proliferation,P53 protein accumulation at cells from papillary and non-papillary regions, and distribution of eosinophilic cell infiltration in human esophagus with immunohistochemistry and CAS 200 Image Analysis System, morphometry and histopathology methods. The results showed that with the lesions from normal to basal cell hyperplasia to dysplasia, the height and the number of papillae in lamina propria increased. The number of immunostaining positive cells by proliferating cell nuclear antigen(PCNA)was higher in papillary region cells than that in nonpapillary region. Papillary immunostaining pattern of P53 protein accumulation was frequently observed in the mild basal cell hyperplasia. The distribution of eosinophilic cell infiltration was also correlated with esophageal lesion progression. Hyperplastic basal cells at the papillary region may be the earliest site for esophageal carcinogenesis. Papillary proliferation in terms of increasing height and number may be the earliest response to abnormal basal cell hyperplasia. Eosinophilic cell infiltration may be a protective response of the body to cell proliferation.
出处
《河南医科大学学报》
1995年第2期97-100,共4页
Journal of Henan Medical University
基金
国家自然科学基金
美国NCI联合资助
