摘要
为了提高血吸虫病病因诊断的特异性和敏感性，用慢性日本血吸虫病病人血清筛选以λｇｔｌｌ构建的日本血吸虫中国大陆株成虫ｃＤＮＡ表达库，获得一阳性克隆Ｓｊｇｔ４，其表达产物为１４８０００融合蛋白。插入的ｃＤＮＡ片段经聚合酶链反应（ＰＣＲ）法扩增、纯化后，亚克隆入高表达载体ｐＧＥＸ，获得克隆ＳｊｐＧ５，其表达产物为５８０００融合蛋白，应用免疫印迹技术，其可被９６％（２４／２５）的日本血吸虫病病人血清中特异抗体所识别。
he sera of patients with chronic infection of
Schis-tosoma japonicum (Sj) were used for screening thecDNA expression library constructed
from Sj adultworm, and an antigen-positive clone Sjgt4 which ex-pressed an 148 KD fusion
protein was identified. Theinserted Sj cDNA fragment was amplified by PCR andsubcloned into
the vector PGEX with high level of ex-pression. A positive clone SjpG5 which expressed a
fu-sion protein with a molecular weight of 58 KD was ob-tained. When the fusion protein was
used for detectionof Sj patients’ sera by Western blpt, 24 out of 25 serawere positive , a
positive rate of 96 %.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1995年第6期329-332,共4页
National Medical Journal of China
