摘要
为了获取日本血吸虫特异的cDNA克隆，用日本血吸虫病流行区化疗后再感染人群血清筛选构建于表达载体λt11的日本血吸虫成虫cDNA文库，再以多聚酶链反应（PCR）技术鉴定免疫筛选的阳性cDNA克隆。结果对cDNA表达库中约8．6×104个噬菌斑进行了筛选，初筛时共挑出59个可能的阳性斑，经两次复筛后，有11个噬菌斑仍呈阳性反应。以阳性噬菌斑DNA为模板，经PCR均扩增出一定大小的片段，从564bp到740bp不等。
To obtain valuable Schistosoma japonicum(Sj) antigen clones, an constructed Sj adult worm cDNA libray was screened with serum from reinfected people of Sj endemic area with high level Sj specific IgG4 antibodies.Then positive clones were preliminarily identified by PCR.Of the 8.6×104 phage plaques immunoscreened by human specific IgG4 antibody, 11 are positive with the PCR products' size ranged from 564bp to 740bp.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2000年第2期67-69,共3页
Chinese Journal of Zoonoses
关键词
日本血吸虫病
CDNA
克隆
免疫筛选
Schistosoma japonicum
Reinfected people
Immunoscreening
cDNA clones
