摘要
目的通过研究湿热环境基质金属蛋白酶-9(MMP-9)及转化生长因子-β(TGF-β)在创面愈合过程中的表达变化,探讨它们对创面愈合所起的作用以及两者之间的相互关系,为临床预防和治疗湿热环境下战伤创面提供理论依据。方法建立湿热环境爆炸伤创面的动物模型,收集伤后4、24、48h和5、7、14、21、28d创面渗液。采用明胶酶谱法和ELISA法,分别对创面MMP-9和TGF-β进行检测。结果创面愈合过程中MMP-9和TGF-β有规律性变化,以伤后48h为高峰,TGF-β伤后7d出现第二高峰,伤后2周两者水平下降,并且出现分离现象。创面给予金属蛋白酶抑制剂(TIMP)的干预,在早期无明显作用,但在后期,即伤后2周,可以降低MMP-9、上调TGF-β的水平。结论创面愈合过程中,早期MMP-9和TGF-β水平上升促进了创面细胞的迁移,有利于创面炎性坏死组织的清除,可能是创面愈合的机制之一,而MMP-9水平的过度升高,不利于创面的愈合。合理外用TIMP对促进延迟愈合创面的愈合会取得良好的效果。
Objective To investigate the expression of endogenetic matrix metalloproteinases-9 (MMP-9) and transforming growth factor-β(TGF-β) and their role in the wound healing of blast injury.Methods Rat models of blast injury under a humid and hot environment were established and the effusion from the wound surface was collected at 4,24,48 h and 5,7,14,21 and 28 days after injury,respectively.The contents of MMP-9 and TGF-β in the effusion of the wound were measured by zymography and enzyme-linked immunosorbent assay (ELISA),respectively.Results During the wound healing of blast injury,MMP-9 and TGF-β exhibited changes that followed a regular pattern,both reaching the peak value at 48 h after the injury.TGF-β content reached the another peak on day 7.TGF-β value and MMP-9 contents decreased in the second week after injury and their reduction was no longer parallel.Administration of tissue inhibitor of metalloproteinase (TIMP) in the early phase of injury showed no obvious effect,but during the 2 weeks after the injury,its administration caused decrease in MMP-9 content and increase in TGF-β content in the effusion.Conclusions In the early phase of wound healing,the elevation of MMP-9 and TGF-β accelerated cell migration to promote the clearance of the inflammatory necrosis tissues,which might be one of the wound healing mechanisms.But overexpression of MMP-9 in the wound may hinder wound healing,and appropriate use of TIMP can accelerate the delayed wound healing.
出处
《第一军医大学学报》
CSCD
北大核心
2005年第7期844-846,共3页
Journal of First Military Medical University
基金
全军十五医学科研项目(01MA134)~~
关键词
爆震伤
伤口愈合
金属蛋白酶类
转化生长因子Β
湿热环境
blast injury
wound healing
matrix metalloproteinases
transforming growth factor-beta
high humid and high hot environment
