摘要
采用PDABavendamm平板和PDARB亮蓝平板对36个平菇菌株进行显色试验,得到农平3号、农大1号、推广1号、冀11等4个可以同时产生漆酶和过氧化物酶的平菇品种.对其进行液态产酶试验,结果表明冀11产木质素降解酶类活性较高,该菌株产酶的最佳条件为pH5.5、28℃、接种3个菌塞、100r/min摇床培养.
<Abstrcat>Colorful reactions of 36 strains of Pleurotus ostreatus were studied through PDA-Bavendamm plate and PDA-RB blue plate separately. It is proved that four strains of Pleurotus ostreatus can produce laccase and peroxidase. The result of the fermentation test in liquid showed that the lignocellulolytic enzymes activity of the strain JI-11 was the highest. The optimal conditions for the strain to produce lignocellulolytic enzymes was: pH5.5, 28 ℃, inoculating three strain plugs, culturing under 100r/min.
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2005年第2期28-32,35,共6页
Journal of Henan University of Technology：Natural Science Edition
基金
河南省杰出人才创新基金资助项目(321001300)
河南省教育厅自然科学基金项目(2003180061)
关键词
平菇
木质素降解酶类
筛选
产酶条件
Pleurotus ostreatus
lignocellulolytic enzymes
screening
enzyme production conditions
