摘要
【目的】深入研究红侧耳双核菌株降解稻草的过程,为开发利用富含木质纤维素的秸秆资源奠定基础。【方法】对BS02、BS09、BS15和BS17等4株红侧耳双核菌株降解稻草过程中,纤维素酶、MnP酶、LiP酶和漆酶的酶活及木质素与纤维素降解率、粗蛋白含量和稻草表面结构的变化等指标进行了测定与分析。【结果】4株红侧耳双核菌株酶活的变化与其对稻草木质纤维素的降解率之间具有一定的相关性,BS17的纤维素酶活、纤维素降解率和粗蛋白产率较高,BS09的MnP、LiP酶活和木质素降解率较高,BS15的漆酶酶活较高。利用扫描电子显微镜对降解稻草秸秆的表面结构进行观察,可看到该菌株主要降解稻草的薄壁细胞,使其发生严重皱缩。【结论】红侧耳在农作物秸秆的综合利用方面具有良好的应用前景。
【Objective】The degradation process of the straw lignocellulose was established with Pleurotus djamor dikaryotic strains.【Method】The activity of cellulose,MnP,LiP and Laccase were measured and the degradation rate of lignin and cellulose,the content of crude protein and the surface structure of straw were analyzed in the degradation process of BS02,BS09,BS15 and BS17.【Result】The certain relativity between change of enzymes by 4 bacterial strain and the degradation rate of straw lignocelluloses was obvious.Th...
出处
《西北农林科技大学学报(自然科学版)》
CSCD
北大核心
2010年第8期204-210,共7页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家农业部重点项目(08zd0101)
西南科技大学青年基金项目(07ZX3124)
关键词
红侧耳
纤维素酶
木质素降解酶类
稻草
秸秆资源利用
Pleurotus djamor
cellulose
lignin degradation enzyme
straw
utilization of straw resources