摘要
目的:建立测定哈蟆油中的十六烷酸胆甾醇酯的含量方法。方法:采用ZorbaxSilica色谱柱,流动相为环己烷-乙醚(40:1);检测波长203nm;柱温40℃;流速1mL·min-1。结果:十六烷酸胆甾醇酯在0.60-8.92μg具有良好线性关系,平均回收率为98.4%,RSD1.8%,具较好的精密度及稳定性。结论:HPLC测定哈蟆油中的十六烷酸胆甾醇酯的含量,不仅操作简单、准确,而且重复性好。
Objective: To develop a new method for the determination of cholesteryl palmitate in Oviductus Ranae. Method: A HPLC method was set up, using Zorbax Silica column and cyclohexane-diethyl ether (40:1) as mobile phase with a flow rate of 1.0 mL·min-1, and the UV detection wavelength was 203 nm. Result: The calibration curve was linear over the range of 0.60-8.92 μg( r = 0.999 7) , the average recovery of the method was 98.4% . RSD 1.8% ( n = 6) . Conclusion: The results showed that method was reliable and accurate.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2005年第13期990-991,共2页
China Journal of Chinese Materia Medica
基金
国家中医药管理局科研基金项目(02-03ZP26)
关键词
哈蟆油
十六烷酸胆甾醇酯
HPLC
含量
Oviductus Ranae
cholesteryl palmitate
content determination
HPLC
