摘要
目的研究慢性血吸虫病患者对乙型肝炎(乙肝)疫苗保护性免疫的影响。方法随机筛选无乙肝病毒感染的慢性血吸虫病患者(实验组)24例,健康志愿者(对照组)26例。分别收集实验组和对照组接种乙肝疫苗前后血清,用ELISA法检测两组接种前后血清乙肝病毒表面抗体(抗-HBs)、肿瘤坏死因子-α(TNF-α)及白细胞介素-2(IL-2)的水平。结果接种乙肝疫苗前实验组和对照组抗-HBs平均吸光度(A值)分别为0.134和0.150。接种后,实验组血清抗-Hbs平均A值为0.217,转阳率为17%,与接种前相比差异无统计学意义(P>0.05);对照组抗-Hbs平均A值为1.210,转阳率为92%,与接种前相比差异有显著的统计学意义(P<0.01)。实验组接种乙肝疫苗前血清IL-2和TNF-α平均水平均明显高于对照组,接种后两组血清IL-2和TNF-α平均水平均升高,但与接种前相比差异无统计学意义(P>0.05)。结论慢性血吸虫病患者对乙肝疫苗的免疫应答水平低下。
Objective To study the impact of chronic schistosomiasis on the protective immunity induced by vaccine against hepatitis B virus. Methods 24 patients with chronic or advanced schistosomiasis (experimental group) and 26 healthy volunteers (control group) all without hepatitis B virus infection were selected for the study. Sera of the subjects in the two groups were collected before inoculation and on the 35th day after inoculation with yeast-derived recombinant hepatitis B vaccine. The level of anti-Hbs, IL-2 and TNF-α in sera was examined by ELISA respectively. Results Anti-Hbs in both groups were negative before inoculation, with an average absorbance (A value) of 0.134 and 0.150 respectively. After inoculation, positive rate of anti-Hbs was 17% (4/24, average A value 0.145 ) in experimental group and 92% (24/26, average A value was 1.210) in control group. The vaccine against hepatitis B induced significantly higher level of anti-Hbs in healthy volunteers compared with that in schistosomiasis patients (P < 0.01). The level of IL-2 and TNF-α increased in both groups after inoculation without significant difference compared with the level before inoculation. Conclusion The results suggest that the protective immunity of patients with chronic schistosomiasis is deficient to the stimulation of hepatitis B virus and it may involve in a higher incidence of hepatitis B among schistosomiasis patients.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2005年第3期163-165,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
湖北省血吸虫病专家咨询委员会资助课题~~
