摘要
目的探讨左旋咪唑(LMS)对细粒棘球蚴感染鼠的免疫调节作用和对病程转归的影响。方法昆明种小鼠腹腔接种细粒棘球蚴,建立感染动物模型。在感染后2、4、8、12、16、20周,未治疗组和实验组分别给予生理盐水和左旋咪唑(25mg/kg),连续7d,测定各组小鼠脾指数、胸腺指数及囊重,运用流式细胞术(FCM)测定小鼠脾CD4+和CD8+T细胞百分率及CD4+/CD8+比值的动态变化。另设8只未接种小鼠为健康对照。结果生理盐水组小鼠感染2周时CD4+、CD8+T细胞百分率显著高于健康对照组(P<0.01)),随后CD4+T细胞的百分率逐渐降低,CD8+T细胞的百分率逐渐增高,至感染后16周和20周,CD4+T细胞、CD8+T细胞的百分率及CD4+/CD8+比值与健康对照组比较差异仍具有显著性(P<0.01)。与生理盐水组小鼠相比,左旋咪唑组小鼠在感染2周时CD4+、CD8+T细胞的百分率明显增高,与健康组小鼠相比有统计学意义(P<0.01);在感染16周和20周时脾指数升高,CD4+T细胞百分率上升,CD8+T细胞的百分率下降,CD4+/CD8+比值升高,平均囊重下降,与生理盐水组比较差异均有显著性(P<0.01)。结论左旋咪唑对细粒棘球蚴感染小鼠有免疫调节作用,在感染后期可升高小鼠脾指数,使CD4+T细胞及CD8+T细胞比例恢复正常,机体的细胞免疫功能增强,从而减缓细粒棘球蚴的增殖。
Objective To explore the immunological regulative effect of levamisole（LMS） on mice infected with Echinococcus granulosus and the turnover of disease. Methods After abdominal inoculation of E. granulosus, mice were divided into NS group and LMS group, then respectively treated with normal saline and LMS（25 mg/kg） for 7 days at 2, 4, 8, 12, 16, 20 week after infection . We researched the percentage of CD4^＋ T cell, CD8^＋ T cell and CD4^＋/ CD8^＋ using FCM and estimate spleen exponent, thymus exponent, average weight of cyst at these different times. And, another 8 healthy mice were as control group. Results As compared with control group, the percentage of CD4 ^＋ T cell and CD8 ^＋T cell of NS group showed a significant increase（P〈0.01） after 2 weeks of post infection（p, i）, then the percentage of CD4 ^＋T cell decreased and the percentage of CD8 ^＋T cell increased gradually. After 16 and 20 weeks of p. i, the percentage of CD4 ^＋ T cell, CD8 ^＋ T cell and CD4^＋/CD8 ^＋showed prominent difference （P〈0.01） compared with NS group. As compared with NS group, the percentage of CD4 ^＋ T cell and CD8^＋ T cell of LMS group increased clearly after 2 weeks of p. i, and after 16 weeks of p. i the percentage of CD4^＋ T cell and CD4^＋/CD8 ^＋increased, the percentage of CD8^＋ T cell and the average weight of cyst decreased in LMS group（P〈0.01）, they all showed prominent difference （P〈0.01） compared with NS group. Conclusion Levamisole can regulate the immune response of mice infected with E. granulosus. In later period of infection it can increase spleen exponent of mice, cause CD4^＋ / CD8 ^＋ back to the normal level, strengthen the immunity of mice, then abate the proliferation of E. granulosus.
出处
《中国病原生物学杂志》
CSCD
2007年第1期57-61,共5页
Journal of Pathogen Biology
