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ABC法检测孢子丝菌病病理组织中病原体的研究 被引量:3

Detection of Organisms in Pathologic Tissues of Sporotrichosis with ABC Technique
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摘要 用ABC-ELISA方法对皮肤抱子丝菌病病理组织中病原体的检测进行了研究。孢子被染成棕褐色,呈圆形、梨形,可见发芽孢子及星状体、巨噬细胞内及白细胞内被吞噬的孢子。孢子直径为2.5um~10.0um。本方法灵敏度为96.2%,特异度为100%。检查1例需要3小时。故认为本方法可用于快速诊断孢子丝菌病及本病的回顾性研究。 The organisms in pathologic tissues of sporotrichosis were detected by ABC technique. Brown spores were found, some spores appeared to be round, or pearshaped,budding spores or asteriod hotlies were observed, and some spores were phagocytlzed by leukocytes and macrophages. The diameter of spores ranged from 2. spin to 10. 0um. The sensitivity of this method is 96. 2% and the specificity 100%. Examining one case takes about three hours. The method can be used for retrospective study, as well as rapid diagnosis of sporotrichosis.
出处 《中华皮肤科杂志》 CAS CSCD 北大核心 1994年第6期345-346,T001,共3页 Chinese Journal of Dermatology
关键词 孢子丝菌病 病理组织 病原体 ABC法 Sporotrichosis
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  • 1吴绍熙,郭宁如,吕桂霞.特比萘芬治疗皮肤孢子丝菌病的研究[J].临床皮肤科杂志,1994,23(5):264-265. 被引量:11
  • 2季荣红.淋球菌五种检测方法的比较[J].中原医刊,2005,32(5):42-42. 被引量:1
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  • 8Sawada H,Aoki K.Rapid diagnosis of enterovirus 70 infection by detection of IgM antibodies with a biotin-avidin ELISA[J].Nippon Ganka Gakkai Zasshi,1990,94(11):1057. 被引量:1
  • 9Moe CL,Allen JR,Monroe SS,et al.Detection of astrovirus in pediatric stool samples by immunoassay and RNA probe[J].J Clin Microbiol,1991,29(11):2390. 被引量:1
  • 10Marques MEA,Coelho KIR,Sotto MN,et al.Comparison between histochemical and immunohistochemical methods for diagnosis of sporotrichosis[J].J Clin Pathol,1992,45:1089. 被引量:1

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