摘要
目的研究人脐动脉血管平滑肌细胞的培养方法。方法运用贴块法进行人脐动脉血管平滑肌细胞的培养,并用倒置显微镜进行形态学观察和用免疫组化对培养细胞进行鉴定。结果运用贴块法成功培养了人脐动脉血管平滑肌细胞,培养的细胞呈典型“峰谷”样生长,免疫组化SP法检测α平滑肌肌动蛋白单克隆抗体(αSMActin)表达,确认为人血管平滑肌细胞。经传代培养至3～5代,细胞生长特性未见异常改变。结论贴块法培养的人脐动脉血管平滑肌细胞生长稳定性好,且培养与纯化能同步进行。
Objective To approach the method of cell culture for vascular smooth muscle cells from umbilical artery.Methods The explant attached method was applied for cell culture of umbilical artery smooth muscle cells .Morphology of the cells were observe by inverted microscope, and identified by immunohistochemical methods.Results The umbilical artery smooth muscle cells was cultured by explant attached method successfully.The cultured umbilical artery grew showed typical 'hill and valley' pattern and identified by the immunohistochemical S-P method(α-SMActin). The umbilical artery vascular smooth muscle cells had no obvious changes of biogic characteristics after 3～5 gengeration of the culture.Conclusions The umbilical artery vascular smooth muscle cells may have a good stability through the explant attached method. The umbilical artery vascular smooth muscle cells could also be cultured and purified simultaneously.
出处
《遵义医学院学报》
2005年第2期118-119,122,共3页
Journal of Zunyi Medical University
基金
贵州省科学技术基金重点项目(黔基合计字(2002)3013号)
关键词
脐动脉
血管平滑肌细胞
细胞培养
umbilical artery
vascular smooth muscle cell
cell culture
