摘要
营养液培养试验,设缺硼、钼和加硼、钼处理,培养后的草坪草海滨雀稗进行2周适温和零上低温处理,研究低温下其活性氧代谢及硼、钼对活性氧代谢的影响。结果表明,与缺硼、钼相比,加硼、钼处理极显著提高低温下草坪草超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性,极显著降低过氧化物酶(POD)活性,极显著降低超氧阴离子(O-·2)产生速率,显著降低质膜电解质相对渗透率,有降低丙二醛(MDA)含量的趋势。而在常温下施硼、钼作用不如低温下明显。在低温下,草坪草超氧阴离子水平极显著升高,MDA含量升高,组织电解质相对外渗率增大,SOD、CAT、POD显著下降表明草坪草已遭受低温寒害,而施硼、钼改善了上述生理生化状况,提高了草坪草抗寒力。
Metabolism of active oxygen species (AOS) in Seashore paspalum (Paspalum vaginatum Swartz.) with or without boron and molybdenum (-BMo, +BMo) were studied with solution culture under low and mild temperature for understanding the possible roles of boron and molybdenum in enhancing the chilling resistance of turfgrass. The results showed that the activities of superoxide dismutase (SOD) and catalase (CAT) were significantly (P<0.01) increased, the activity of peroxidase (POD) was significantly (P<0.01) decreased, the rate of active oxygen species (O^(-·)_2)(P<0.01) and membrane permeability were significantly(P<0.05) decreased, and the content of malondialdehyde (MDA) was trended towards decrease by the application of B and Mo under low temperature. The activity of SOD,POD and CAT decreased significantly (P<0.05), while O^(-·)_2 producing rate and malondialdehyde (MDA) content increased significantly (P<0.01), and membrane permeability increased under low temperature, suggesting that the turfgrass was subjected to chilling stress. Thus it indicates that boron and molybdenum could enhance the chilling resistance of turfgrass by protecting the plant from the injury of AOS via increasing activities of scavenging enzymes.
出处
《作物学报》
CAS
CSCD
北大核心
2005年第6期755-759,共5页
Acta Agronomica Sinica
基金
广东省佛山市科技发展专项资金资助项目(0102010A)。
关键词
海滨雀稗
抗寒力
活性氧
硼
钼
Seashore paspalum (Paspalum vaginatum Swartz.)
Chilling resistance
Active oxygen species
Boron
Molybdenum
