摘要
目的 研究血管生成因子1(angiopoietin -1,Ang -1)和2(Ang- 2)在头颈鳞状细胞癌(简称鳞癌)及健康黏膜组织中的表达及意义。方法 采用免疫组化法,观测了45例头颈部鳞癌组织和7例健康志愿者口腔黏膜组织中Ang -1和Ang- 2的表达及分布。分析了不同临床分期及不同病理分级的癌组织中Ang- 1和Ang -2表达水平及其意义。结果 Ang -1和Ang- 2在血管内皮细胞、健康黏膜上皮细胞及鳞癌细胞内均有表达;Ang -1在肿瘤的血管内皮细胞及癌细胞的阳性表达率分别为78% (35 /45)和87% (39 /45),Ang 2在肿瘤的血管内皮细胞及癌细胞的阳性表达率分别为69% (31 /45)和76% (34 /45),均高于健康黏膜的血管内皮细胞及上皮细胞内的表达(P<0 .05);肿瘤血管内皮细胞与鳞癌细胞内Ang-1、Ang -2的表达水平有关(P<0 .05);Ang- 1在肿瘤血管内皮细胞中的表达水平与肿瘤的临床分期有关,在ⅢⅣ期比在ⅠⅡ期高(P<0 .05);Ang -2在肿瘤的血管内皮细胞及鳞癌细胞中的表达也表现为ⅢⅣ期比ⅠⅡ期高(P<0. 05);Ang -1及Ang- 2的表达水平在高、中、低分化的癌组织中的差异无统计学意义(P>0 .05)。结论 肿瘤血管内皮细胞与鳞癌细胞内Ang- 1、Ang -2的表达水平有关,且分别高于健康黏膜的血管内皮细胞及上皮细胞的表达。晚期肿瘤的血管生成与Ang -
Objective To study the significance by analyzing the expression of angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) in the tissues of squmaous cell carcinoma (SCC) of the head and neck areas and in normal mucosa tissues. Methods The expression of Ang-1 and Ang-2 in 45 tumor samples and 7 normal mucosa tissues were determined by the immunohistochemical method with avidin-biotin-peroxidase complex technique. The results were scored by two independent observers and analyzed statistically. Results The positive expression of Ang-1 and Ang-2 existed in the endothelial cells, epithelial cells and also in SCC cells. The positive expression rates of Ang-1 in tumor samples was 78% in the endothelial cells, and 87% in SCC cells. The positive expression rates of Ang-2 in tumor samples was 69% in the endothelial cells, and 76% in SCC cells. The scores of positive expression of Ang-1 and Ang-2 were higher in endothelial cells and in SCC cells of tumor tissues than that of normal mucosa tissues (rank sum test, P<0.05). There was positive correlation between the expression of Ang-1 and Ang-2 in the endothelial cells and also in SCC cells (Chi-square test with contingency table, P<0.05). Ang-1 expression in endothelial cells of tumor tissues was higher in clinical stage Ⅲ-Ⅳ than that in clinical stage Ⅰ-Ⅱ (rank sum test, P<0.05). Ang-2 expression in both endothelial cells and SCC cells, were higher in clinical stage Ⅲ-Ⅳ than that in clinical stage Ⅰ-Ⅱ (rank sum test, P<0.05). There was no statistical significance for degrees of Ang-1 and Ang-2 expression in different histological grades(P>0.05). Conclusions The expressions of Ang-1 and Ang-2 in advanced SCC were remarkable. Ang-1 and Ang-2 may play a critical role during the progress of SCC of head and neck areas.
出处
《中华耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2005年第5期371-375,共5页
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
基金
中国留学基金委资助项目(99831079)
德国JeanUhrmacherScholarshipinCologneofGermany资助
