摘要
目的 探讨苯并 (a)芘 [B(a)P]对鲫鱼全脑一氧化氮 (NO)和一氧化氮合酶 (NOS)的影响及水生态毒理学机制 ,同时为苯并 (a)芘的水污染监测探寻灵敏简便的亚致死指标。方法 设立空白对照、溶剂对照、3× 1 0 -4～ 3×1 0 -1 mg/LB(a)P处理组共 6个实验组 ,分别于染毒后 1 ,3,6和 1 2d取鲫鱼全脑进行检测。 结果 全脑一氧化氮(NO)、一氧化氮合酶 (NOS)水平与苯并 (a)芘存在着剂量及染毒时间依赖关系。结论 引起体内NOS活性升高及NO的过量产生是苯并 (a)芘在鲫鱼体内发挥毒性的机制之一。
Objective To study the effect of B(a)P on nitric oxide(NO) and nitric oxide synthase (NOS) activity in brain of carassius auratus,and to explore the toxic mechanism of B(a)P in aquatic ecotoxicology.Meanwhile,to find sensitive and convenient sublethal biomarkers. Methods Six groups were incorporated,including blank control,carrier control,and 3×10 -4 -3×10 -1 mg/L B(a)P groups.After an exposure of 1,3,6?d and 12?d respectively,determining the biomarkers.Results NO level and NOS activity had a dose-and time-dependent change after exposure to B(a)P.Conclusion The induction of NOS and NO was one of the means by which B(a)P damaged carassius auratus.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2005年第2期186-187,共2页
Chinese Journal of Public Health
基金
山东省科委基金资助
