摘要
目的通过观察旋转刺激下大鼠行为学变化,鉴别大鼠运动病判断指标的特异性及判别效率。方法用Crampton模型旋转刺激SD大鼠不同时间后,观察异嗜高岭土行为、条件性厌食行为、2h摄食量及探究行为(旷场试验)的变化。结果大鼠不同时间旋转刺激后,与刺激前相比高岭土摄入量明显增加,糖精水摄入量、旷场试验积分、2h摄食量则明显减少;糖精水摄入量、旷场试验积分、2h摄食量随着刺激时间的延长而逐渐下降,与刺激时间呈指数曲线关系;高岭土摄入量在旋转运动刺激12h以内,随时间延长而逐渐增加,且与刺激时间呈直线关系,刺激18h高岭土摄入量反而低于刺激6h和12h,呈下降趋势。旋转刺激12h内,高龄土摄入量、糖精水摄入量、2h摄食量、旷场试验积分与时间的校正相关系数依次为:0.94、0.54、0.44及0.34。旋转刺激6h各个指标的判断效率最高,分别为:异嗜高岭土70%;条件性厌食90%;2h摄食量80%;旷场试验积分95%。结论异嗜高岭土和条件性厌食是判断运动病发生的较为特异的指标,以6h为最佳旋转刺激时间,综合运用包括异嗜行为、条件性厌食行为、2h摄食量和旷场试验积分等行为学指标,有利于提高大鼠运动病易感性和严重程度判断的准确性,是较为可靠的运动病行为学实验方法。
Objective To assess the specification and efficiency of ratation sickness indices by monitoring changes of behaviors in rats under rotation stimulation. Method SD rats were stimulated by Crampton model with different time courses. Pica or kaolin consumption (KC), conditioned taste aversion (CTA) or saccharine water ingestion (SWI), 2 h food ingestion (2hFI), and open-field test (OFT) scores were observed. Result Apparent changes of the four indices were observed after rotation stimulation. SWI, OFT scores and 2hFI decreased exponentially with increase of duration of the motion stimulation. KC increased linearly with the increase of time within 12 h stimulation. After 18 h stimulation, KC decreased to a level even lower than that after 6 or 12 h stimulation. The adjusted correlation between changes of the indices and duration of stimulation within 12 h are : 0.94 for KC, 0.54 for SWI, 0.44 for 2hFI and 0.34 for OFT. The maximum efficiency of the four indices appeared at 6-hour stimulation: 70% for KC, 90% for SWI, 80% for 2hFI and 95% for OFT. Conclusion It is found that pica and CTA were more specific than the other indices. They may serve as primary indices and can be combined with the secondary indices such as 2hFI or OFT. Six hours is the optimal duration of stimulation by Crampton model for rotation sickness studies.
出处
《航天医学与医学工程》
CAS
CSCD
北大核心
2005年第2期98-101,共4页
Space Medicine & Medical Engineering
关键词
运动病
异嗜行为
条件性厌食
旷场试验
糖精水
motion sickness
pica
conditioned anorexia
open-field test
saccharine water
