摘要
采用溶胶-凝胶法制备了纳米普鲁士蓝微粒,将含纳米普鲁士蓝微粒的TiO2溶胶-凝胶固定在玻碳电极表面得到纳米普鲁士蓝修饰电极,该电极对H2O2产生灵敏的响应,线性范围为0.5～400 μmol/L,较常规普鲁士蓝修饰电极(线性范围为25～500 μmol/L)灵敏.电极表面再用溶胶-凝胶法固定葡萄糖氧化酶后构建了葡萄糖生物传感器,响应范围0～20 mmol/L,葡萄糖氧化酶表观米氏常数为8.04 mmol/L.实验表明,该法适合于批量制作高灵敏和高重现性的生物传感器.
Electrodes were fabricated by printing of dioxide titanic sol-gel containing Prussian blue nanoparticles on glassy carbon surface. The electrode gave higher sensitivity with a linear range of response to H2O2 of 0. 5 ∼ 400 μ mol/L than normal Prussian blue modified electrode (linear range of 25 ∼ 500 μ mol/L). Glucose sensors were constructed by glucose oxidase ( GOD) immobilized on the electrode. The resulting biosensors showed amperometric responses to glucose with a linear range of 0 ∼ 20 mmol/L. The apparent Michaelis-Menten constant K-m is 8. 04 mmol/L. The result showed that this method could be applied to manufacture high sensitivity and repeatability biosensors.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2005年第4期538-542,共5页
Chinese Journal of Analytical Chemistry
基金
广西教育厅科研基金项目资助(桂教科研[2003]22
[2004]20)
