摘要
目的建立人类前列腺癌裸鼠原位肿瘤模型,为前列腺癌的研究提供有用的工具.方法将2×106PC-3细胞注射于10只BALB/c裸小鼠背部靠近腋窝处.8周后,取出背部肿瘤,剪成小块,种植于20只裸鼠前列腺背侧叶被膜下,缝合包埋固定.9～12周后处死裸鼠,对前列腺和相关器官进行检测,确定肿瘤生长和转移情况.结果18只(90%)裸鼠前列腺生长出肿瘤,17只肿瘤直径＞1.5 cm.12只因梗阻出现膀胱扩张和肾积水,10只出现腹膜后主动脉旁淋巴结转移,4只出现肺转移,1只肝转移,无骨转移.病理切片可见大部分腺体被肿瘤细胞破坏,细胞胞核浓染,呈多形性,可见异常分裂相;转移淋巴结的皮质和髓质被肿瘤细胞占据.结论外科原位移植技术建立的前列腺癌模型,较好地保留了肿瘤细胞的生物学习性,生长快,局部侵犯范围较广,有较高的淋巴转移和肺转移率,是较理想的前列腺癌异种移植模型.
Objective To establish an orthotopic animal model of human prostate carcinoma (PC),and to provide a useful tool for the research of PC. Methods A total of 30 male BALB/c nude mice were included.First,2×10 6 human PC-3 cells were injected subcutaneously in 10 nude mice.Then 8 weeks later,the tumor on mice backs were took out and smashed into pieces.The tissue was implanted on the ventral lateral lobe of prostate gland by surgical orthotopic implantation in the other 20 nude mice.Twelve weeks later, the mice were autopsied,and histopathological examination of orthotopic tumors and relevant organs was done to identify and quantitate micrometastasis. Results Of the 20 mice,18 (90%) had tumor growth.The tumors were greater than 1.5 cm in diameter in 17 mice.Dilatation of bladder and hydronephrosis because of obstruction occurred in 12 mice.A high frequency of lymph node and organ metastasis was noted upon histological examination (10 with retroperitoneal para-aortic lymph node metastasis,4 with lung metastasis,1 with liver metastasis).But no bone metastasis was found. Pathological sections showed that most parts of the prostate were occupied by tumor cells. The nuclei looked polymorphic and hyperchromatic.Abnormal nucleus division could be seen.The cortices and medullae of metastatic lymph nodes were occupied by tumor cells. Conclusions The model set up by surgical orthotopic implantation can show the biological features of prostate carcinoma cells well.It expresses the metastatic potential of PC cell line significantly and can be considered an ideal heterotransplant animal model.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2005年第3期208-209,共2页
Chinese Journal of Urology
基金
国家自然科学基金资助项目(No. 32120665)
