摘要
对１２个品种的二棱大麦（Ｈｏｒｄｅｕｍｄｉｓｔｉｃｈｕｍ２ｎ＝２４）幼穗和６个品种的幼胚在附加５００ｍｇ／Ｌ酪蛋白，１５０ｍｇ／Ｌ天门冬素和２ｍｇ／Ｌ２，４－Ｄ的ＭＳ培养基（Ｍ＿（３２０））中进行了离体培养，并诱导产生出了愈伤组织。把由Ｄ＿１幼胚所获得的愈伤组织转移到含２，４－Ｄ０．５ｍｇ／Ｌ（Ｍ＿（３０５））培养基中后诱导产生出了体细胞胚性愈伤组织，幼苗和根。筛选出了体细胞胚性无性系。在长达二年的继代培养中，该细胞系仍保持旺盛的再生和分化成苗能力。
variety immature spike and 6 varietal young embryo of Hordeum distichum,2n= 24,were cultured on MS medium with 500 mg/L caseinhydrolysate,150 mg/L L-Asparagineand 2 mg/L2,4-D(M_(320)).The callus began to be formed.Trasplanted calli from young em-bryo of D_1 on MS medium with 0.5mg/L 2,4-D(M_(305))were induced to produce somatic em-bryogenesis,buds and roots,and embryonic somaclones were selected. Their regeneration wasremained highly after long-term subcuIture capability for 2 years.
出处
《西北植物学报》
CAS
CSCD
北大核心
1994年第1期14-18,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
陕西省1992年自然科学基金
关键词
二棱大麦
无性系
大麦
体细胞
胚
Hordeum distichum,immature spike and embryo,callus,somatic embryo,embry-onic somaclones.
