摘要
撕去下表皮的豌豆(Pisum satium L.)幼嫩叶片置于含1％纤维素酶(EA_8-867)和20mM CaCl_2·2H_2O的0.6M甘露醇溶液中,于25+1℃条件下酶解4—6小时可获得大量具活力的原生质体。原生质体置于含1.0—1.5mg/L 6-BA和0.5—1.0mg/L 2,4—D的本研究所确定的培养基中进行了培养。8天后即可重新生壁,4天后出现第一次细胞分裂,7天后出现二次分裂,然后持续分裂。一个月后形成了肉眼可见的愈伤组织。比较研究了影响原生质体分离、培养和形成愈伤组织的各种因素。
The young leaves of Pisum satium L. torn the lower epidermis were incubated in an enzyme solution consisting of 1% cellulase EA_3-867, 20mM CaCl_2.2H_2O and 0.7M mannitol for 4-6 hours at 25±1℃. Numerous viable protoplasts were cultured on the medium defined in this study supplemented with 1.0—1.5 mg/L 6-BA and 0.5—1.0mg/L 2,4—D.These protoplasts regenerated new cell walls after 3 days in culture, The first cell division occurred after 4 days. The second cell division occurred after 7 days of culture. Then the cells divided continously. After a mouth of culture, small calli formed and could be seen with naked eyes. We also studied comparatively various factors affecting isolation, culture and callus formation from mesophyll cell protoplasts of pea.
出处
《西北植物学报》
CAS
CSCD
北大核心
1990年第4期269-274,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
杨陵农业科技开发基金
关键词
豌豆
原生质体
愈伤组织
Pisum satium L.
Mesophyll protoptasts
Cullus
